Wang Mengge, Chen Xiaofeng, Wang Zhigang, Li Ming
College of Environmental Science and Engineering, Yangzhou University, Yangzhou, 225127, People's Republic of China.
Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, Zhoushan Municipal Center for Disease Control and Prevention, Zhoushan, 316021, People's Republic of China.
Anal Sci. 2025 Mar 20. doi: 10.1007/s44211-025-00744-6.
Ionic liquids (ILs) which belong to the molten salt of a high ionic environment exhibit a number of unique properties, including the formation of various heterogeneities in their microemulsion. We found that auto-generated radicals within a [Bmim][PF]/[Cmim][PF]/PBS reverse micelles (IL-RMs) and then the radicals were quantified in this study. For the radical confirmation, EPR spectroscopy with 5,5-dimethyl-1-pyrroline N-oxide (DMPO) free radical scavenger was carried out. A spectroscopic chemical probe of 1,1-diphenyl-2-picrylhydrazine (DPPHH) was employed to quantify the radicals in the IL-RM systems. The mechanism of the radical generation was also proposed. The DPPHH probe method is simple, rapid, and sensitive to quantify free radicals in the IL-RM systems. Finally, IL-RMs were successfully applied to degrade Rhodamine B (Rho-B) dye with low degradation cost, simple operation, short time consumption, and remarkable degradation effect. The results show a near 100% degradation rate for 10 μmol·L Rho-B in the IL-RMs system with pH 7.4 PBS containing 1 mmol·L chloroacetic acid as an aqueous phase.
离子液体（ILs）属于高离子环境的熔盐，具有许多独特的性质，包括在其微乳液中形成各种不均匀性。我们发现在[Bmim][PF]/[Cmim][PF]/PBS反胶束（IL-RMs）中会自动产生自由基，并且在本研究中对这些自由基进行了定量。为了确认自由基，使用5,5-二甲基-1-吡咯啉N-氧化物（DMPO）自由基清除剂进行电子顺磁共振光谱分析。采用1,1-二苯基-2-苦基肼（DPPHH）光谱化学探针来定量IL-RM系统中的自由基。还提出了自由基产生的机制。DPPHH探针法简单、快速且灵敏，可用于定量IL-RM系统中的自由基。最后，IL-RMs成功应用于降解罗丹明B（Rho-B）染料，具有降解成本低、操作简单、耗时短和降解效果显著的特点。结果表明，在含有1 mmol·L氯乙酸作为水相的pH 7.4 PBS的IL-RMs系统中，10 μmol·L Rho-B的降解率接近100%。
