Lanave Gianvito, Pellegrini Francesco, Diakoudi Georgia, Catella Cristiana, Cavalli Alessandra, Capozza Paolo, Elia Gabriella, Di Martino Barbara, Zini Eric, Pollicino Giuseppe, Zatelli Andrea, Bányai Krisztián, Lavazza Antonio, Decaro Nicola, Camero Michele, Martella Vito
Department of Veterinary Medicine, University of Bari Aldo Moro, Valenzano, Bari, Italy.
Department of Veterinary Medicine, University of Teramo, Teramo, Italy.
Sci Rep. 2025 Mar 20;15(1):9650. doi: 10.1038/s41598-025-94123-w.
Two feral cats (from the same colony) were presented to the veterinary clinic for weakness, weight loss, and anorexia. The cats were part of a study on feline hepatotropic viruses (collection A, 43 animals). On metaviromic investigation, parvoviral reads were identified in the sera of the two cats. The feline parvovirus genome was 5.3 kb long with an organization similar to other members of the Erythroparvovirus genus. In the ORF1 (nonstructural proteins) and ORF2 (VP1/VP2 precursor) the feline virus displayed 43.1% and 49.1% nucleotide identity to human parvovirus B19, and 48.9% and 56.6% to chipmunk parvovirus. Sequence identity to canine/feline protoparvovirus (Protoparvovirus carnivoran 1) was as low as 36.5% % and 29.2% in the ORF1 and ORF2, respectively. Using a quantitative PCR assay, the virus was also identified in an additional ten cats (prevalence 27.6%, 12/43) from collection A and in 15/1150 (1.3%) of archival sera (collection B), revealing a higher infection rate in cats with altered hepatic markers, suggestive of hepatic distress. The findings of our study extend the list of known parvoviruses in the feline host.
两只流浪猫（来自同一群落）因身体虚弱、体重减轻和食欲不振被送到兽医诊所。这两只猫是一项关于猫嗜肝病毒研究（A组，共43只动物）的一部分。在宏病毒组研究中，在这两只猫的血清中鉴定出细小病毒序列。猫细小病毒基因组长度为5.3 kb，其结构与红细胞细小病毒属的其他成员相似。在开放阅读框1（非结构蛋白）和开放阅读框2（VP1/VP2前体）中，该猫病毒与人类细小病毒B19的核苷酸同源性分别为43.1%和49.1%，与花栗鼠细小病毒的同源性分别为48.9%和56.6%。在开放阅读框1和开放阅读框2中，其与犬/猫原细小病毒（食肉动物原细小病毒1）的序列同源性分别低至36.5%和29.2%。使用定量PCR检测，在A组另外10只猫（患病率27.6%，12/43）以及1150份存档血清（B组）中的15份（1.3%）中也检测到了该病毒，这表明肝脏指标异常的猫（提示肝脏功能障碍）感染率更高。我们的研究结果扩展了猫宿主中已知细小病毒的种类。
