Que Hongrui, Wang Yuxuan, Feng Yi, Tu Shaoqin, Wei Jiaming, Cheung Chiyuen, Wei Nan, Chen Zheng, Ai Hong
The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
Department of Stomatology, Shenzhen Sixth People'S Hospital (Nanshan Hospital) Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, People's Republic of China.
BMC Neurosci. 2025 Mar 20;26(1):26. doi: 10.1186/s12868-025-00945-9.
To explore the effect and mechanism of hippocampus on experimental orthodontic pain-induced anxiety.
Herein, we document a novel modeling method whereby the nickel-titanium (Ni-Ti) orthodontic wire was fixed stably in the oral cavity of mice with a ligation technique to induce stable distal movement of maxillary incisors to mimic orthodontic tooth movement. At the experimental endpoint, serum corticosterone assay, Golgi staining and Micro-CT were performed in each group after oral-facial mechanical pain sensitivity assessment and open field test.
The mechanical pain sensitivity of experimental tooth movement pain (ETMP) mice had an apparent increased elicited following tooth movement. And anxiety-like behavior was developed: reduced the time proportion of center zone and the total moving distance in the open field test and the elevated serum corticosterone levels in ETMP mice relative to control group mice. The Golgi staining in ventral hippocampal CA1 revealed that neural spine density, dendritic length and number of dendrites are reduced markedly in ETMP mice compared with the control group.
Experimental orthodontic pain drives emotional anxiety through the plasticity changes in decreased neuronal complexity and reduced spine density in ventral hippocampal CA1 in mice.
探讨海马体对实验性正畸疼痛诱导的焦虑的影响及机制。
在此，我们记录了一种新型建模方法，即通过结扎技术将镍钛（Ni-Ti）正畸丝稳定固定在小鼠口腔中，以诱导上颌切牙稳定向远中移动，模拟正畸牙齿移动。在实验终点，在每组进行口腔面部机械性疼痛敏感性评估和旷场试验后，进行血清皮质酮测定、高尔基染色和显微CT检查。
实验性牙齿移动疼痛（ETMP）小鼠在牙齿移动后机械性疼痛敏感性明显增加。并且出现了焦虑样行为：在旷场试验中，ETMP小鼠相对于对照组小鼠，中心区域时间比例和总移动距离减少，血清皮质酮水平升高。腹侧海马CA1区的高尔基染色显示，与对照组相比，ETMP小鼠的神经棘密度、树突长度和树突数量明显减少。
实验性正畸疼痛通过小鼠腹侧海马CA1区神经元复杂性降低和棘密度减少的可塑性变化导致情绪焦虑。
