Characterization and comparison of temperature-sensitive mutants.

The F-BAR protein Cdc15 is essential for cytokinesis in the fission yeast , playing a key scaffolding role and connecting the actomyosin-based cytokinetic ring to the plasma membrane. Here, we compared temperature-sensitive mutants isolated in multiple genetic screens. We determined the mutations within each mutant allele and analyzed their growth at different temperatures. Additionally, we report a new allele that highlights the requirement for Cdc15 in the recruitment of the early secretory pathway to the cellular division site. The new mutants described here expand the toolkit for studying cytokinesis in .