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有丝分裂 F-BAR 蛋白 Cdc15 的无规则结构域在维持有丝分裂环完整性方面发挥着独特的重要功能。

The intrinsically disordered region of the cytokinetic F-BAR protein Cdc15 performs a unique essential function in maintenance of cytokinetic ring integrity.

机构信息

Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37240.

出版信息

Mol Biol Cell. 2019 Oct 15;30(22):2790-2801. doi: 10.1091/mbc.E19-06-0314. Epub 2019 Sep 11.

Abstract

Successful separation of two daughter cells (i.e., cytokinesis) is essential for life. Many eukaryotic cells divide using a contractile apparatus called the cytokinetic ring (CR) that associates dynamically with the plasma membrane (PM) and generates force that contributes to PM ingression between daughter cells. In important membrane-CR scaffolds include the paralogous F-BAR proteins Cdc15 and Imp2. Their conserved protein structure consists of the archetypal F-BAR domain linked to an SH3 domain by an intrinsically disordered region (IDR). Functions have been assigned to the F-BAR and SH3 domains. In this study we probed the function of the central IDR. We found that the IDR of Cdc15 is essential for viability and cannot be replaced by that of Imp2, whereas the F-BAR domain of Cdc15 can be swapped with several different F-BAR domains, including that of Imp2. Deleting part of the IDR results in CR defects and abolishes calcineurin phosphatase localization to the CR. Together these results indicate that Cdc15's IDR has a nonredundant essential function that coordinates regulation of CR architecture.

摘要

两个子细胞(即胞质分裂)的成功分离对生命至关重要。许多真核细胞使用称为细胞分裂环(CR)的收缩装置进行分裂,该装置与质膜(PM)动态相关联,并产生力,有助于 PM 在子细胞之间向内侵入。在重要的膜-CR 支架中包括同源的 F-BAR 蛋白 Cdc15 和 Imp2。它们保守的蛋白质结构由典型的 F-BAR 结构域通过一个固有无序区域(IDR)与 SH3 结构域相连。已经为 F-BAR 和 SH3 结构域分配了功能。在这项研究中,我们研究了中央 IDR 的功能。我们发现 Cdc15 的 IDR 对生存至关重要,不能被 Imp2 的 IDR 取代,而 Cdc15 的 F-BAR 结构域可以与几个不同的 F-BAR 结构域(包括 Imp2 的 F-BAR 结构域)交换。删除 IDR 的一部分会导致 CR 缺陷,并使钙调磷酸酶定位到 CR 上。这些结果表明 Cdc15 的 IDR 具有非冗余的基本功能,可协调 CR 结构的调节。

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