Khaldoun Hassina, Settar Amina, Oularbi Yasmine, Boudjema Nouara, Amokrane Assia, Djennane Nacima, Tarzaali Dalila
Department of Biology, Faculty of Nature and Life Sciences, University of Blida 1, Route de Soumaa, BP270, Blida, Algeria.
Department of Agri-food, Faculty of Nature and Life Sciences, University of Blida 1, Route de Soumaa, BP270, Blida, Algeria.
Toxicol Rep. 2025 Feb 10;14:101959. doi: 10.1016/j.toxrep.2025.101959. eCollection 2025 Jun.
The increasing use of pesticides has raised concerns about their gastrointestinal toxicity, leading to the search for natural remedies such as thyme essential oil. For that, this study aimed to determine the protective effect of Thymus vulgaris essential oil (TEO) with its chemical composition against Voliam Targo-induced duodenal toxicity. Twenty male rabbits were randomly assigned to four equal groups and treated for 21 consecutive days: Control, VT insecticide group, TEO essential oil group, and VT + TEO group. The main constituent of the essential oil of T. vulgaris was carvacrol 72.9 %. The duodenal injury was assessed using biochemical, histomorphometrical, and immunohistochemical methods. The VT induced an increased number of benign intestinal tissue changes, such as hyperplasia of Brunner glands, disorganization of villi, and infiltration of inflammatory cells. The co-administration of TEO with VT restored the histological organization of the duodenum. In addition, the immunohistochemical examination of the duodenal tissues shows positive immunostaining for the expression of Ki67, P53, and BCL2 proteins in the VT group. Lower expressions were noted in the VT-TEO group compared to the control and TEO groups. The E-cadherin and β-catenin immuno-signals were significantly higher in the essential oil treatment groups' duodenal sections than in the VT group. The study suggested that VT caused duodenal toxicity and that the carvacrol chemotype of TEO could mitigate and alleviate this effect.
农药使用的增加引发了人们对其胃肠道毒性的担忧，促使人们寻找诸如百里香精油之类的天然疗法。为此，本研究旨在确定百里香精油（TEO）及其化学成分对Voliam Targo诱导的十二指肠毒性的保护作用。将20只雄性兔子随机分为四组，连续21天进行治疗：对照组、VT杀虫剂组、TEO精油组和VT + TEO组。百里香精油的主要成分是香芹酚，含量为72.9%。采用生化、组织形态计量学和免疫组织化学方法评估十二指肠损伤。VT导致良性肠道组织变化的数量增加，如Brunner腺增生、绒毛紊乱和炎性细胞浸润。TEO与VT联合使用恢复了十二指肠的组织学结构。此外，十二指肠组织的免疫组织化学检查显示，VT组中Ki67、P53和BCL2蛋白表达呈阳性免疫染色。与对照组和TEO组相比，VT - TEO组的表达较低。精油治疗组十二指肠切片中的E - 钙黏蛋白和β - 连环蛋白免疫信号明显高于VT组。该研究表明，VT会导致十二指肠毒性，而TEO的香芹酚化学型可以减轻和缓解这种影响。
