Abdulqader Saeed, Kaya Abdullah, Marif Hardi, Ali Basim, Ismaeel Dana
Biara Veterinary Centre, Directorate of Veterinary in Sulaimani, Biara, Kurdistan Region, Iraq.
Department of Internal Medicine, Faculty of Veterinary Medicine, University of Yuzuncu Yil, Van, Turkiye.
Vet Med (Praha). 2025 Feb 26;70(2):45-53. doi: 10.17221/60/2024-VETMED. eCollection 2025 Feb.
The parasitic protozoan causes cryptosporidiosis in young calves, leading to diarrhoea and financial losses in the farming industry. This study aimed to examine the occurrence of in preweaning calves suffering from diarrhoea in Sulaymaniyah, Iraq, using both enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) methods. Faecal samples were obtained from 80 young calves categorised into various groups according to age, breed, sex, and geographic origin. Notably, a greater occurrence of infection was observed in female calves, those in the 5-30 days age group, and those of the Friesian breed. Furthermore, the highest infection rate was reported in the Zarayan region. A strong correlation was observed between the ELISA and PCR findings. The molecular analysis detected both and , with documented for the first time in Iraq. infection considerably affects physiological indicators, particularly in younger calves, including body temperature, heart rate, and respiratory rate. PCR positivity in our study was substantially correlated with dehydration. Overall, this study highlights the need for prompt identification and intervention for the management of infections in young calves.
这种寄生原生动物会导致幼小牛患隐孢子虫病，引发腹泻并给养殖业造成经济损失。本研究旨在采用酶联免疫吸附测定（ELISA）和聚合酶链反应（PCR）方法，检测伊拉克苏莱曼尼亚患腹泻的断奶前小牛体内该原生动物的感染情况。从80头幼小牛采集粪便样本，这些小牛根据年龄、品种、性别和地理来源分为不同组。值得注意的是，在雌性小牛、5至30日龄组的小牛以及弗里西亚品种的小牛中观察到该感染的发生率更高。此外，扎拉扬地区报告的感染率最高。ELISA和PCR结果之间观察到强相关性。分子分析检测到了该原生动物的两种类型，其中一种在伊拉克首次记录到。该原生动物感染对生理指标有显著影响，尤其是在幼小牛中，包括体温、心率和呼吸频率。在我们的研究中，PCR阳性与脱水密切相关。总体而言，本研究强调了对幼小牛感染进行及时识别和干预以进行管理的必要性。
