Yang Yiting, Zhang Yao, Zhang Wenying, Lu Kunpeng, Wang Liping, Liu Yanfang, Du Linna, Yang Jing, Guan Lili, Ma Hongxia
College of Life Sciences, Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, P. R. China.
Affiliated Hospital of Changchun University of Chinese Medicine, Changchun 130021, P. R. China.
J Agric Food Chem. 2025 Apr 2;73(13):7788-7806. doi: 10.1021/acs.jafc.4c12105. Epub 2025 Mar 21.
This study elucidated the mechanisms underlying the immunoregulatory and gut-microbiota-modulating effects of residue polysaccharide (FVRP) using cyclophosphamide (CTX)-induced mouse models. FVRP supplementation alleviated CTX-induced intestinal damage and boosted antioxidant enzyme activity and cytokine secretion. Additionally, FVRP enhanced the diversity and total species richness of the gut microbiota, promoting the proliferation of beneficial bacteria (e.g., ), while reducing the abundance of CTX-derived bacteria ( and ). FVRP facilitates the accumulation of short-chain fatty acids. Untargeted metabolomic analyses of cecal content revealed that FVRP treatment notably restored the levels of 32 endogenous metabolites altered by CTX. Based on a pseudosterility mice model, fecal microbiota transplantation (FMT), and fecal filtrate transplantation (FFT), gut microbiota and associated metabolites were demonstrated to play a crucial role in the immunomodulatory and protective effects of FVRP against intestinal injury. In conclusion, FVRP exhibits significant potential as an immune enhancer and natural therapeutic agent for alleviating intestinal inflammatory conditions.
本研究利用环磷酰胺（CTX）诱导的小鼠模型，阐明了残渣多糖（FVRP）免疫调节和肠道微生物群调节作用的潜在机制。补充FVRP可减轻CTX诱导的肠道损伤，提高抗氧化酶活性和细胞因子分泌。此外，FVRP增强了肠道微生物群的多样性和总物种丰富度，促进了有益细菌（如 ）的增殖，同时降低了CTX衍生细菌（ 和 ）的丰度。FVRP促进短链脂肪酸的积累。盲肠内容物的非靶向代谢组学分析表明，FVRP处理显著恢复了CTX改变的32种内源性代谢物的水平。基于无菌小鼠模型、粪便微生物群移植（FMT）和粪便滤液移植（FFT），肠道微生物群和相关代谢物被证明在FVRP对肠道损伤的免疫调节和保护作用中起关键作用。总之，FVRP作为一种免疫增强剂和缓解肠道炎症的天然治疗剂具有巨大潜力。
