College of Life Sciences, Engineering Research Center of Bioreactor s Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, PR China.
Changchun Gaorong Biotechnological Co., Ltd., Changchun 130102, PR China.
Int J Biol Macromol. 2024 Nov;281(Pt 4):136487. doi: 10.1016/j.ijbiomac.2024.136487. Epub 2024 Oct 14.
Flammulina velutipes residues (FVR) are the waste culture medium derived from the collection of Flammulina velutipes fruiting bodies, with an annual output that remains largely unexplored. The characteristics of digestion and fermentation of Flammulina velutipes residues polysaccharide (FVRP) are still relatively unknown. This study investigated the structure of the gut microbiota through 16 s rDNA gene sequencing and analyzed changes in short-chain fatty acid (SCFA) content via targeted metabolome analysis. The aim was to explore the prebiotic activity of FVRP based on a simulated digestion model combined with an in vitro anaerobic fermentation model. The results demonstrated that FVRP did not exhibit significant changes during in vitro digestion and fermentation but did enhance antioxidant activity. Furthermore, FVRP was found to rapidly reduce the pH value and increase SCFA production in the fermentation broth from lactic acid bacteria and human feces. Notably, FVRP altered the gut microbiota structure, significantly increasing the relative abundance of Firmicutes and Bacteroidota. Thus, FVRP could be considered a promising prebiotic food and feed additive that promotes the generation of short-chain fatty acids by modulating gut microbiota.
金针菇菌渣（FVR）是金针菇子实体收获后产生的废弃栽培基质，其年产量尚未得到充分开发。金针菇菌渣多糖（FVRP）的消化和发酵特性仍知之甚少。本研究通过 16s rDNA 基因测序探究肠道微生物群的结构，并通过靶向代谢组分析来分析短链脂肪酸（SCFA）含量的变化。旨在通过模拟消化模型结合体外厌氧发酵模型，探索 FVRP 的益生元活性。结果表明，FVRP 在体外消化和发酵过程中没有发生明显变化，但能增强抗氧化活性。此外，FVRP 能迅速降低发酵液的 pH 值并增加来自乳酸菌和人粪便的发酵液中 SCFA 的生成。值得注意的是，FVRP 改变了肠道微生物群的结构，显著增加了厚壁菌门和拟杆菌门的相对丰度。因此，FVRP 可以被认为是一种有前途的益生元食品和饲料添加剂，通过调节肠道微生物群来促进短链脂肪酸的生成。
