da Silva Elaine Cristina, Paes de Brito Leandro, Oliveira Silva Francisca Crislândia, da Silva Santos Dayane, Andrade Mendonça Allyson, Bezerra Raquel Pedrosa, Galindo Soares Paulo Antônio, de Morais Junior Marcos Antônio, Figueiredo Porto Ana Lúcia, Holanda Cavalcanti Maria Taciana
Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil.
Federal University of Pernambuco, Recife, Pernambuco, Brazil.
Prep Biochem Biotechnol. 2025;55(6):787-796. doi: 10.1080/10826068.2025.2479829. Epub 2025 Mar 21.
Cheese whey (CW) represents a potential substrate in biotechnological processes due to the presence of valuable nutrients in its composition. Therefore, CW is used as a low-cost substrate in fermentation for microbial growth and the synthesis of value-added compounds, while mitigating the environmental impact that this by-product can cause. The current study aimed to obtain exopolysaccharides (EPS) by fermenting lactic acid bacteria in CW followed by optimizing production using Response Surface Methodology (RSM) and evaluating the biological properties. Out of 64 isolates, sp. 133 V exhibited a high concentration with 5.58 mg/mL of EPS. With optimization using RSM, 21.74 mg/mL of EPS was obtained with temperature and fermentation time fixed at 42 °C and 22 h, respectively. The characterization of the new EPS revealed a hetero-polysaccharide consisting of galactose, glucose, mannose, arabinose, rhamnose and fucose, including proteins and uric acid in the structure. With a concentration of 2 mg/mL, the purified EPS showed good scavenging effects against DPPH (27%), ABTS (72%) and superoxide (43%), except for the hydroxyl radical (1.29%) which needs a high EPS concentration. These findings underscore the interest in using cheap residue as culture medium to produce biopolymers with potential for applications, particularly in the food and biotechnology sectors.
由于奶酪乳清（CW）的成分中含有有价值的营养物质，它在生物技术过程中是一种潜在的底物。因此，CW被用作发酵中的低成本底物，用于微生物生长和增值化合物的合成，同时减轻这种副产品可能造成的环境影响。当前的研究旨在通过在CW中发酵乳酸菌来获得胞外多糖（EPS），随后使用响应面法（RSM）优化生产并评估其生物学特性。在64株分离株中，sp. 133 V表现出较高的EPS浓度，为5.58 mg/mL。通过使用RSM进行优化，在温度和发酵时间分别固定为42 °C和22 h的情况下，获得了21.74 mg/mL的EPS。新EPS的表征显示其为一种杂多糖，由半乳糖、葡萄糖、甘露糖、阿拉伯糖、鼠李糖和岩藻糖组成，结构中还包括蛋白质和尿酸。在浓度为2 mg/mL时，纯化后的EPS对DPPH（27%）、ABTS（72%）和超氧阴离子（43%）表现出良好的清除效果，但对羟基自由基的清除效果（1.29%）需要较高的EPS浓度。这些发现凸显了利用廉价残渣作为培养基生产具有应用潜力的生物聚合物的意义，特别是在食品和生物技术领域。
