Akbari Nazanin, Tafazoli Shaghayegh, Heidari Banafsheh
Department of Biology, Shahid Beheshti University, Tehran, Iran.
Clinical Research Development Unit, Ghaem Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Cell Tissue Bank. 2025 Mar 21;26(2):17. doi: 10.1007/s10561-025-10166-3.
Chronic diabetic wounds, such as diabetic foot ulcers, pose a significant health challenge due to their prolonged healing times and high recurrence rates. Conventional treatments are often inadequate, driving interest in advanced therapeutic approaches like biological scaffolds. Decellularized scaffolds, which replicate the extracellular matrix (ECM), have shown potential in promoting tissue regeneration and wound healing. This study evaluated the efficacy of decellularized bovine articular cartilage scaffolds in enhancing wound healing in a preclinical murine model of chronic diabetic wounds. Bovine articular cartilage was decellularized using a combination of chemical and physical processes. The scaffolds were characterized through H and E staining (to assess histomorphological characteristics), FTIR, and SEM analyses to confirm ECM preservation and effective decellularization. Twenty female diabetic BALB/c mice were divided into two groups: a control group (treated with Atrauman Ag® dressings) and an experimental group (treated with decellularized bovine articular cartilage scaffolds). This study examined the effects of decellularization on the structural and chemical properties of the cartilage scaffolds, as well as their impact on wound healing and closure rates in diabetic mice compared to the control group. Mice treated with the decellularized cartilage scaffolds demonstrated a significantly faster wound closure rate (100% closure by day 17) compared to the control group (75% closure by day 17, P < 0.01). Histological analysis revealed more organized epidermal regeneration, fibrin deposition, and granulation tissue formation in the scaffold-treated group. SEM and FTIR analyses confirmed the preservation and integrity of the ECM before and after the decellularization process. Decellularized bovine articular cartilage scaffolds significantly enhance wound healing in chronic diabetic wounds by promoting tissue regeneration and reducing inflammation. These findings suggest that such scaffolds represent a promising therapeutic option for the treatment of chronic diabetic wounds.
慢性糖尿病伤口，如糖尿病足溃疡，因其愈合时间长和复发率高而构成重大的健康挑战。传统治疗方法往往效果不佳，这激发了人们对生物支架等先进治疗方法的兴趣。脱细胞支架可复制细胞外基质（ECM），已显示出促进组织再生和伤口愈合的潜力。本研究评估了脱细胞牛关节软骨支架在慢性糖尿病伤口临床前小鼠模型中促进伤口愈合的效果。采用化学和物理方法相结合的方式对牛关节软骨进行脱细胞处理。通过苏木精和伊红染色（以评估组织形态学特征）、傅里叶变换红外光谱（FTIR）和扫描电子显微镜（SEM）分析对支架进行表征，以确认细胞外基质的保存情况和有效的脱细胞效果。将20只雌性糖尿病BALB/c小鼠分为两组：对照组（用Atrauman Ag®敷料治疗）和实验组（用脱细胞牛关节软骨支架治疗）。本研究考察了脱细胞处理对软骨支架结构和化学性质的影响，以及与对照组相比，其对糖尿病小鼠伤口愈合和闭合率的影响。与对照组（第17天75%闭合，P < 0.01）相比，用脱细胞软骨支架治疗的小鼠伤口闭合速度明显更快（第17天100%闭合）。组织学分析显示，支架治疗组的表皮再生更有序，有纤维蛋白沉积和肉芽组织形成。SEM和FTIR分析证实了脱细胞处理前后细胞外基质的保存和完整性。脱细胞牛关节软骨支架通过促进组织再生和减轻炎症，显著增强了慢性糖尿病伤口的愈合。这些发现表明，此类支架是治疗慢性糖尿病伤口的一种有前景的治疗选择。
