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从小鼠骨髓细胞生成和评估功能性巨噬细胞的方案。

Protocol for the generation and assessment of functional macrophages from mouse bone marrow cells.

作者信息

Scafidi Andrea, Lind-Holm Mogensen Frida, Michelucci Alessandro

机构信息

Neuro-Immunology Group, Department of Cancer Research, Luxembourg Institute of Health, 1210 Luxembourg, Luxembourg; Faculty of Science, Technology and Medicine, University of Luxembourg, 4365 Esch-sur-Alzette, Luxembourg.

Neuro-Immunology Group, Department of Cancer Research, Luxembourg Institute of Health, 1210 Luxembourg, Luxembourg; Faculty of Science, Technology and Medicine, University of Luxembourg, 4365 Esch-sur-Alzette, Luxembourg.

出版信息

STAR Protoc. 2025 Mar 20;6(2):103706. doi: 10.1016/j.xpro.2025.103706.

DOI:10.1016/j.xpro.2025.103706
PMID:40120113
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11976235/
Abstract

Macrophages play essential roles in tissues, wherein they exert beneficial or detrimental functions depending on the signals they encounter during their differentiation. Here, we present a protocol to differentiate mouse bone marrow cells into macrophages under specific cues to evaluate their impact on macrophage phenotypic acquisition. We provide detailed instructions for optimal cell culture conditions, quality controls, and examples of concluding immunological functional assays. This protocol is applicable in short- and long-term drug-based modulation of macrophage functions. For complete details on the use and execution of this protocol, please refer to Scafidi et al..

摘要

巨噬细胞在组织中发挥着至关重要的作用，在分化过程中，它们根据所遇到的信号发挥有益或有害的功能。在此，我们展示了一种在特定条件下将小鼠骨髓细胞分化为巨噬细胞的方案，以评估其对巨噬细胞表型获得的影响。我们提供了关于最佳细胞培养条件、质量控制的详细说明，以及总结免疫功能测定的示例。该方案适用于基于药物的巨噬细胞功能的短期和长期调节。有关该方案使用和执行的完整详细信息，请参考斯卡菲迪等人的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/d9b86976167e/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/1500750f937b/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/2ba678160505/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/ff945a2fff44/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/87d37ea7c388/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/093f097f5f23/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/804cfa94760c/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/837ad46c89b8/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/d2731bd07b47/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/e79d2d1457bd/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/b79de7686caa/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/937ed76eeaf6/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/d9b86976167e/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/1500750f937b/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/2ba678160505/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/ff945a2fff44/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/87d37ea7c388/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/093f097f5f23/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/804cfa94760c/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/837ad46c89b8/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/d2731bd07b47/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/e79d2d1457bd/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/b79de7686caa/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/937ed76eeaf6/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccf8/11976235/d9b86976167e/gr11.jpg

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