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小鼠牙槽骨髓造血干细胞分离与鉴定方案

Protocol for the isolation and characterization of mouse alveolar bone marrow hematopoietic stem cells.

作者信息

Niizuma Kouta, Morikawa Satoru, Gars Eric, Chang Alyssa H, Wilkinson Adam C, Nakauchi Hiromitsu, Yamamoto Ryo

机构信息

Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA.

Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Dentistry and Oral Surgery, Keio University School of Medicine, Tokyo, Japan.

出版信息

STAR Protoc. 2025 Jun 20;6(2):103875. doi: 10.1016/j.xpro.2025.103875. Epub 2025 Jun 5.

DOI:10.1016/j.xpro.2025.103875
PMID:40482037
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12173640/
Abstract

Here, we present a protocol for isolating and characterizing hematopoietic stem cells (HSCs) from mouse alveolar bone marrow. We describe steps for combining anatomical dissection of the mandibular alveolar region with flow cytometry analysis for identifying HSCs. The protocol enables the isolation of HSCs from a specific bone marrow niche harboring an enriched HSC population. For complete details on the use and execution of this protocol, please refer to Niizuma et al..

摘要

在此，我们展示了一种从小鼠牙槽骨骨髓中分离和鉴定造血干细胞（HSCs）的方案。我们描述了将下颌牙槽区域的解剖 dissection 与用于鉴定 HSCs 的流式细胞术分析相结合的步骤。该方案能够从含有丰富 HSC 群体的特定骨髓生态位中分离出 HSCs。有关此方案的使用和执行的完整详细信息，请参考 Niizuma 等人的研究。 （注：原文中“anatomical dissection”直译为“解剖 dissection”，这里“dissection”可能是多余的词汇，正常应是“解剖”，整体翻译时保留了原文表述。）

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dad/12173640/a52004a7e17d/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dad/12173640/29b08c043722/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dad/12173640/47cf2b7d6380/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dad/12173640/a52004a7e17d/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dad/12173640/29b08c043722/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dad/12173640/47cf2b7d6380/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dad/12173640/a52004a7e17d/gr2.jpg

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本文引用的文献

1
Elevated hematopoietic stem cell frequency in mouse alveolar bone marrow.小鼠牙槽骨髓中造血干细胞频率升高。
Stem Cell Reports. 2025 Jan 14;20(1):102374. doi: 10.1016/j.stemcr.2024.11.004. Epub 2024 Dec 12.
2
Mapping the immune microenvironment for mandibular alveolar bone homeostasis at single-cell resolution.以单细胞分辨率绘制下颌牙槽骨稳态的免疫微环境图谱。
Bone Res. 2021 Mar 15;9(1):17. doi: 10.1038/s41413-021-00141-5.
3
Long-term ex vivo expansion of mouse hematopoietic stem cells.长期的体外扩增小鼠造血干细胞。
Nat Protoc. 2020 Feb;15(2):628-648. doi: 10.1038/s41596-019-0263-2. Epub 2020 Jan 8.
4
Long-term ex vivo haematopoietic-stem-cell expansion allows nonconditioned transplantation.长期的体外造血干细胞扩增可实现非条件性移植。
Nature. 2019 Jul;571(7763):117-121. doi: 10.1038/s41586-019-1244-x. Epub 2019 May 29.
5
Changing concepts in hematopoietic stem cells.造血干细胞概念的转变
Science. 2018 Nov 23;362(6417):895-896. doi: 10.1126/science.aat7873.
6
Hoxb5 marks long-term haematopoietic stem cells and reveals a homogenous perivascular niche.Hoxb5标记长期造血干细胞并揭示一个同质的血管周围微环境。
Nature. 2016 Feb 11;530(7589):223-7. doi: 10.1038/nature16943.