Shi Huan, Li Pingbo, Yun Peng, Zhu Yun, Zhou Hao, Wang Lu, Wu Bian, Wang Yipei, Lou Guangming, Huang Qin, Gao Guanjun, Zhang Qinglu, Chen Junxiao, Li Jinbo, Xiao Jinghua, You Aiqing, He Yuqing
National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.
Institute of Food Crop, Hubei Academy of Agricultural Science, Wuhan, China.
Plant Biotechnol J. 2025 Jun;23(6):2367-2382. doi: 10.1111/pbi.70064. Epub 2025 Mar 22.
Grain shape and tiller angle are two important agronomic traits influencing grain yield and quality in rice. Herein, we map-based cloned a grain shape gene GLW9 (Grain Length and Width on chromosome 9), which encodes a DNA binding with one finger (DOF) family transcription factor OsDOF25. GLW9 positively regulates grain length and negatively regulates grain width, consequently improving grain length-to-width ratio and appearance quality. GLW9 binds to the EXPA6 promotor to upregulate its expression, thereby positively regulating cell expansion and grain shape. On the other hand, GLW9 directly upregulates the expression of OsPIN1b to reduce tiller angle. This study elucidates the mechanism by which GLW9 coordinately regulates grain shape and tiller angle, providing theoretical reference and gene resources for the improvement of grain shape and tiller angle in rice.
粒形和分蘖角度是影响水稻产量和品质的两个重要农艺性状。在此，我们通过图位克隆法分离出一个粒形基因GLW9（位于第9号染色体上的粒长和粒宽基因），它编码一个单指DNA结合（DOF）家族转录因子OsDOF25。GLW9正向调控粒长，负向调控粒宽，从而提高长宽比和外观品质。GLW9与EXPA6启动子结合以上调其表达，从而正向调控细胞扩张和粒形。另一方面，GLW9直接上调OsPIN1b的表达以减小分蘖角度。本研究阐明了GLW9协同调控粒形和分蘖角度的机制，为改良水稻粒形和分蘖角度提供了理论参考和基因资源。
