NasrEldeen Mohammed S, Ragab Refaat S A, Aboelmaaty Amal M, Saudi Eltaher M
Theriogenology Department, Faculty of Veterinary Medicine, Cairo UniversityGiza. Address. PO Box 12211 - Giza, Cairo - Egypt and Department of Surgery & Theriogenology, Faculty of Veterinary Medicine, King Salman International University, Ras Sudr - Obour Street- King Salman International University Tur Sinai - South of Sinaa, 46612, Egypt; Theriogenology Department, Faculty of Veterinary Medicine, Cairo University- Giza. Address. PO Box 12211 - Giza, Cairo, Egypt.
Theriogenology Department, Faculty of Veterinary Medicine, Cairo UniversityGiza. Address. PO Box 12211 - Giza, Cairo - Egypt and Department of Surgery & Theriogenology, Faculty of Veterinary Medicine, King Salman International University, Ras Sudr - Obour Street- King Salman International University Tur Sinai - South of Sinaa, 46612, Egypt.
Domest Anim Endocrinol. 2025 Jul;92:106941. doi: 10.1016/j.domaniend.2025.106941. Epub 2025 Mar 11.
For exploring the protective effects of selenium (SeNPs) and zinc (ZnNP) nanoparticles on testicular blood flow, morphometry, oxidants-antioxidant status, reproductive hormones, and blood biochemicals of goats subjected to heat stress, bucks (n=15) were equally divided into control, ZnNP (50.0 mg/animal), and SeNPs (0.57 mg/animal) groups. Bucks were supplemented daily for seven weeks during summer. The testicular artery diameter, PSV, EDV, TAMV, Mean V, RI, PI, BFV, testicle length, width, height, epididymal length, and width were determined. Blood samples were collected for measuring LH, testosterone and estradiol, zinc, selenium, NO, MDA, SOD, TAC, CAT, total proteins, albumin, AST, and ALT. Results indicated that testicular volume, mediastinum testes, height, epididymal tail length, and width increased (P<0.0001) in animals supplemented with ZnNPs. Testicular (P<0.05) and epididymal tail (P<0.01) width increased during the supplementation. SeNPs and ZnNPs decreased (P<0.0001) the testicular artery diameter and increased PSV (P<0.05), and V Mean (P<0.01). ZnNPs increased TAMV (P<0.05) and BFV (P<0.01). RI (P<0.01), PI (P<0.05), and BFV (P<0.01) decreased after withdrawing supplementation associated with increased (P<0.05) testicular artery diameter. Sperm mass and individual motility, live sperm %, sperm cell concentration, and SPMI Improved (P<0.0001) in ZnNPs and SeNPs groups during and after the supplementation. ZnNPs and SeNPs decreased CAT (P<0.0001) and testosterone with increasing SOD (P<0.05), globulin, and ALT. SeNPs increased (P<0.0001) estradiol and LH. In conclusion, ZnNPs or SeNPs improved the semen parameters during heat stress by modulating testicular blood flow, testicular thermoregulatory mechanism, and antioxidant status, proving nontoxic to the liver and kidneys.
为探究硒纳米颗粒（SeNPs）和锌纳米颗粒（ZnNP）对热应激山羊睾丸血流、形态计量学、氧化-抗氧化状态、生殖激素及血液生化指标的保护作用，将15只公羊平均分为对照组、ZnNP组（50.0毫克/只）和SeNPs组（0.57毫克/只）。在夏季，对公羊进行为期7周的每日补充。测定睾丸动脉直径、峰值流速（PSV）、舒张末期流速（EDV）、平均加速度（TAMV）、平均流速（Mean V）、阻力指数（RI）、搏动指数（PI）、血流速度（BFV）、睾丸长度、宽度、高度、附睾长度和宽度。采集血样以检测促黄体生成素（LH）、睾酮和雌二醇、锌、硒、一氧化氮（NO）、丙二醛（MDA）、超氧化物歧化酶（SOD）、总抗氧化能力（TAC）、过氧化氢酶（CAT）、总蛋白、白蛋白、天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）。结果表明，补充ZnNPs的动物睾丸体积、睾丸纵隔、高度、附睾尾长度和宽度增加（P<0.0001）。补充期间睾丸（P<0.05）和附睾尾（P<0.01）宽度增加。SeNPs和ZnNPs使睾丸动脉直径减小（P<0.0001），PSV增加（P<0.05），平均流速（V Mean）增加（P<0.01）。ZnNPs使TAMV增加（P<0.05），BFV增加（P<0.01）。停止补充后，RI（P<0.01）、PI（P<0.05）和BFV（P<0.01）降低，同时睾丸动脉直径增加（P<0.05）。在补充期间及补充后，ZnNPs组和SeNPs组的精子质量、个体活力、活精子百分比、精子细胞浓度和精子活力指数均得到改善（P<0.0001）。ZnNPs和SeNPs使CAT降低（P<0.0001），睾酮降低，同时SOD（P<）、球蛋白和ALT增加。SeNPs使雌二醇和LH增加（P<0.0001）。总之，ZnNPs或SeNPs通过调节睾丸血流、睾丸体温调节机制和抗氧化状态，改善了热应激期间的精液参数，且对肝脏和肾脏无毒。 05）
