Ghramh Hamed A, Ibrahim Essam H, El-Arabey Amr Ahmed, Khan Khalid Ali
Central Labs, King Khalid University, AlQura'a, P.O. Box 960, Abha, Saudi Arabia.
Center of Bee Research and Its Products, King Khalid University, P.O. Box 9004, 61413, Abha, Saudi Arabia.
Sci Rep. 2025 Mar 22;15(1):9973. doi: 10.1038/s41598-025-94698-4.
Honey and its derivatives are extensively acknowledged for their diverse biological attributes. Sidr honey, a specific type of monofloral honey, is obtained from the nectar of the Sidr tree (Ziziphus spina-christi). Muslims believe that water from the Zamzam well has the potential to treat a wide range of diseases. Across the globe, millions utilize Zamzam water, which is characterized by its naturally alkaline properties. SidrZamZam honey represents an innovative variant of honey, fundamentally akin to Sidr honey, with the notable difference being that bees are supplied with Zamzam water as their hydration source. The objective of this research was to evaluate the capacity of two distinct varieties of honey to produce silver nanoparticles (AgNPs), examine their antibacterial properties, investigate their impact on immune cells, and assess their anticancer efficacy against luminal A breast cancer (MCF-7). The findings indicated that both Sidr and SidrZamZam honeys possessed the ability to synthesize varying sizes of silver nanoparticles (AgNPs). In this study, AgNPs synthesized by both honeys and incorporated within them demonstrated significant antibacterial properties. In addition, the two varieties of honey, when evaluated independently, displayed anticancer effects against the MCF-7 cancer cell line; however, this was not the case when these honeys were combined with AgNPs. Additionally, both varieties of honey facilitated the proliferation of immune cells. In this context, our bioinformatic analyses demonstrate that several immune cell types exert a clinically significant influence on patients with luminal-A breast cancer. These immune cell types include CD8 T cells, regulatory T cells (T), neutrophils, M0 macrophages, M1 macrophages, M2 macrophages, resting myeloid dendritic cells, resting mast cells, and follicular helper T cells. Therefore, these immune cell subsets represent promising targets for the treatment of luminal-A breast cancer utilizing both varieties of honey. In conclusion, Sidr and SidrZamZam honeys may serve as a source for the environmentally-friendly synthesis of AgNPs. Furthermore, both honey types have the potential to enhance immune cell activity and can function as immunomodulators. Besides, both honeys may hold promise in the combat against breast cancer, pending further research.
蜂蜜及其衍生物因其多样的生物学特性而被广泛认可。锡德蜂蜜是一种特定类型的单花蜂蜜，由锡德树（枣椰树）的花蜜制成。穆斯林认为渗渗泉的水有治疗多种疾病的潜力。在全球范围内，数百万人使用渗渗泉水，其特点是天然呈碱性。锡德渗渗蜂蜜是蜂蜜的一种创新变体，从根本上来说与锡德蜂蜜类似，显著的区别在于蜜蜂被提供渗渗泉水作为其水源。本研究的目的是评估两种不同蜂蜜产生银纳米颗粒（AgNPs）的能力，检测它们的抗菌特性，研究它们对免疫细胞的影响，并评估它们对管腔A型乳腺癌（MCF - 7）的抗癌效果。研究结果表明，锡德蜂蜜和锡德渗渗蜂蜜都具有合成不同尺寸银纳米颗粒（AgNPs）的能力。在本研究中，由两种蜂蜜合成并包含在其中的AgNPs表现出显著的抗菌特性。此外，这两种蜂蜜单独评估时，对MCF - 7癌细胞系显示出抗癌作用；然而，当这些蜂蜜与AgNPs结合时并非如此。此外，两种蜂蜜都促进了免疫细胞的增殖。在此背景下，我们的生物信息学分析表明，几种免疫细胞类型对管腔A型乳腺癌患者具有临床上的显著影响。这些免疫细胞类型包括CD8 T细胞、调节性T细胞（T）、中性粒细胞、M0巨噬细胞、M1巨噬细胞、M2巨噬细胞、静息髓样树突状细胞、静息肥大细胞和滤泡辅助性T细胞。因此，这些免疫细胞亚群是利用这两种蜂蜜治疗管腔A型乳腺癌的有希望的靶点。总之，锡德蜂蜜和锡德渗渗蜂蜜可作为环境友好型合成AgNPs的来源。此外，两种蜂蜜都有增强免疫细胞活性的潜力，并且可以作为免疫调节剂发挥作用。此外，两种蜂蜜在对抗乳腺癌方面可能有前景，但有待进一步研究。
