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小鼠中“原位”激活神经元的标记与操作方案。

Protocol for labeling and manipulating "home"-activated neurons in mice.

作者信息

Su Xiaoya, He Junyue, Liu Yunlong, Liu Keyu, Lei Bo, Zhong Yi

机构信息

Tsinghua-Peking Center for Life Sciences, Beijing 100084, P.R. China; School of Life Sciences, Tsinghua University, Beijing 100084, P.R. China; McGovern Institute of Brain Research, Beijing 100084, P.R. China.

School of Life Sciences, Tsinghua University, Beijing 100084, P.R. China; McGovern Institute of Brain Research, Beijing 100084, P.R. China; Peking University, Tsinghua University, National Institute Biological Science Joint Graduate Program, Beijing, P.R. China.

出版信息

STAR Protoc. 2025 Mar 22;6(2):103704. doi: 10.1016/j.xpro.2025.103704.

DOI:10.1016/j.xpro.2025.103704
PMID:40121664
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11981720/
Abstract

Home-related cues, such as home bedding, influence affective behaviors in mice, including fear relief, anxiety reduction, and defensive aggression. Here, we present a protocol for labeling and manipulating "home"-activated neurons in the lateral hypothalamus (LH) using a Tet-Off inducible system. We describe steps for virus injection, postoperative recovery, and combining c-Fos-driven activity labeling with chemogenetics. This protocol integrates neuronal targeting with behavioral assays to provide insights into the functional role of "home"-activated neurons in home-driven behavioral adaptations. For complete details on the use and execution of this protocol, please refer to Su et al..

摘要

与家相关的线索，如家用品，会影响小鼠的情感行为，包括恐惧缓解、焦虑减轻和防御性攻击。在此，我们展示了一种使用Tet-Off诱导系统标记和操纵下丘脑外侧区（LH）中“家”激活神经元的方案。我们描述了病毒注射、术后恢复以及将c-Fos驱动的活动标记与化学遗传学相结合的步骤。该方案将神经元靶向与行为分析相结合，以深入了解“家”激活神经元在由家驱动的行为适应中的功能作用。有关该方案使用和执行的完整详细信息，请参考Su等人的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ad1/11981720/658bafb2e800/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ad1/11981720/bc2a3cd91cfb/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ad1/11981720/13acf56765d5/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ad1/11981720/18fa8c563b14/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ad1/11981720/8d37fc41adaa/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ad1/11981720/3f3b46cfb2f1/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ad1/11981720/658bafb2e800/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ad1/11981720/bc2a3cd91cfb/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ad1/11981720/13acf56765d5/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ad1/11981720/18fa8c563b14/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ad1/11981720/8d37fc41adaa/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ad1/11981720/3f3b46cfb2f1/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ad1/11981720/658bafb2e800/gr5.jpg

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