Hultman J, Ronquist G
Scand J Thorac Cardiovasc Surg. 1985;19(1):69-76. doi: 10.3109/14017438509102825.
The myocardial contents of pyruvate, lactate and orthophosphate after ischemia were investigated in a paracorporeal rat heart model under different conditions. The arterial blood was supplemented with phosphoenolpyruvate (PEP) and adenosine triphosphate early during reperfusion of excised hearts subjected to 15 min of complete global ischemia at 37 degrees C. The increase in myocardial pyruvate was significant after 4 min of reperfusion compared with the content in non-supplemented hearts subjected to the same ischemic trauma. Such dynamic changes were not observed for lactate and orthophosphate under corresponding conditions. The distinct increase in myocardial pyruvate content on supplementation with PEP and adenosine triphosphate was most probably due to an adenosine triphosphate-mediated PEP translocation into myocardial cells, with rapid metabolization of translocated PEP into adenosine triphosphate (in the presence of cellular adenosine diphosphate) and pyruvate. The pyruvate and lactate relationship varied, depending on the postischemic conditions. The postischemic myocardial orthophosphate content was stable, with only minor fluctuations. This possibility to supply the postischemic myocardium with substrate for immediate intracellular energy production is of clinical interest and merits further studies.
在不同条件下，利用大鼠体外心脏模型研究了缺血后心肌中丙酮酸、乳酸和正磷酸盐的含量。在37℃下对离体心脏进行15分钟完全性全心缺血后再灌注的早期，向动脉血中补充磷酸烯醇丙酮酸（PEP）和三磷酸腺苷。与未补充的心脏在遭受相同缺血损伤后的含量相比，再灌注4分钟后心肌丙酮酸含量显著增加。在相应条件下，乳酸和正磷酸盐未观察到这种动态变化。补充PEP和三磷酸腺苷后心肌丙酮酸含量明显增加，很可能是由于三磷酸腺苷介导PEP转运到心肌细胞内，转运的PEP在细胞二磷酸腺苷存在的情况下迅速代谢为三磷酸腺苷和丙酮酸。丙酮酸和乳酸的关系因缺血后条件而异。缺血后心肌正磷酸盐含量稳定，仅有轻微波动。这种为缺血后心肌提供底物以立即进行细胞内能量产生的可能性具有临床意义，值得进一步研究。
