Hultman J, Ronquist G
Eur Surg Res. 1984;16(4):201-13. doi: 10.1159/000128410.
Infusion of phosphoenolpyruvate together with adenosine triphosphate early during reperfusion after ischemia increased the energy content and the left ventricular isovolumic performance in a paracorporeal rat heart model. Excised hearts were subjected to 15 min of complete global ischemia at 37 degrees C before reperfusion. During reperfusion they were either nonworking (empty-beating) or working (performing left ventricular isovolumic work) between 20 and 40 min of reperfusion before freeze-clamping. Early during reperfusion the excised hearts were either supplemented with 144 mumol phosphoenolpyruvate and 0.67 mumol adenosine triphosphate or nonsupplemented (plain saline) administered in a pulsatile arterial blood flow. The supplemented nonworking group showed significantly higher creatine phosphate and adenosine triphosphate contents compared to the nonsupplemented group. However, under postischemic working conditions there were no significant differences in high-energy phosphates between the supplemented and the nonsupplemented groups in spite of significantly better left ventricular isovolumic performance in the supplemented group. Concomitantly the efflux of creatine kinase isoenzyme MB indicating cellular damage was significantly less for this group. The data presented here favor the view of a possible compartmentalized adenosine triphosphate pool with a high metabolic turnover close to the plasma membrane.
在体外大鼠心脏模型中，在缺血后再灌注早期输注磷酸烯醇丙酮酸和三磷酸腺苷可增加能量含量和左心室等容性能。离体心脏在37℃下经历15分钟的完全全心缺血后再灌注。在再灌注期间，在冷冻钳夹前20至40分钟的再灌注过程中，它们要么不工作（空跳），要么工作（进行左心室等容作功）。在再灌注早期，离体心脏要么补充144 μmol磷酸烯醇丙酮酸和0.67 μmol三磷酸腺苷，要么不补充（生理盐水），以脉动动脉血流给药。补充的不工作组与未补充组相比，磷酸肌酸和三磷酸腺苷含量显著更高。然而，在缺血后工作条件下，尽管补充组的左心室等容性能明显更好，但补充组和未补充组之间的高能磷酸盐没有显著差异。与此同时，该组中表明细胞损伤的肌酸激酶同工酶MB的流出量显著减少。此处呈现的数据支持这样一种观点，即可能存在一个靠近质膜的具有高代谢周转率的三磷酸腺苷区室化池。
