Kaminska Marta, Dudzinska Noemie A M, Yucel-Lindberg Tülay, Söder Birgitta, Narayanan Aswathy, Potempa Jan, Mydel Piotr M
Broegelmann Research Laboratory, Department of Clinical Science, Faculty of Medicine, University of Bergen, Bergen, Norway.
Division of Pediatric Dentistry, Department of Dental Medicine, Karolinska Institutet, Stockholm, Sweden.
J Oral Microbiol. 2025 Mar 20;17(1):2479903. doi: 10.1080/20002297.2025.2479903. eCollection 2025.
(Pg) is a keystone pathogen in periodontitis, encoding a unique peptidyl arginine deiminase (PPAD) linked to protein citrullination, a process associated with rheumatoid arthritis (RA). Recently, we identified a super-active PPAD variant (T2) in isolates. Here, we evaluated if the presence of the super-active T2 variant of PPAD affects the salivary microbiome, the severity of chronic periodontitis (CP), and subsequently CP's causative association with RA onset/progression.
PATIENTS/MATERIALS AND METHODS: We examined 56 CP patients and 36 healthy volunteers. and counts were measured RT-PCR, and PPAD variant was typed PCR. 16S rRNA from salivary DNA sequencing characterized microbiota composition, while CP severity was assessed through bleeding on probing (BoP), clinical attachment loss (CAL), and pocket depth (PD) parameters.
CP patients exhibited higher and counts, with 30.7% harbouring the PPAD-T2 variant, compared to only one healthy volunteer. Clinical CP parameters were unaffected by the PPAD variant. However, PPAD-T2 influenced oral microbiota composition, enriching certain genera.
While the PPAD variant did not affect CP severity, it influenced oral microbiota composition. Further research is needed to understand citrullination's role in oral microbiota and chronic inflammatory disease development.
牙龈卟啉单胞菌（Pg）是牙周炎中的关键病原体，编码一种与蛋白质瓜氨酸化相关的独特肽基精氨酸脱氨酶（PPAD），该过程与类风湿性关节炎（RA）有关。最近，我们在分离株中鉴定出一种超活性PPAD变体（T2）。在此，我们评估了PPAD超活性T2变体的存在是否会影响唾液微生物群、慢性牙周炎（CP）的严重程度，以及随后CP与RA发病/进展的因果关系。
患者/材料与方法：我们检查了56名CP患者和36名健康志愿者。通过逆转录聚合酶链反应（RT-PCR）测量牙龈卟啉单胞菌和伴放线聚集杆菌数量，并通过聚合酶链反应（PCR）对PPAD变体进行分型。对唾液DNA测序得到的16S核糖体RNA进行分析，以确定微生物群组成，同时通过探诊出血（BoP）、临床附着丧失（CAL）和牙周袋深度（PD）参数评估CP严重程度。
与仅一名健康志愿者相比，CP患者的牙龈卟啉单胞菌和伴放线聚集杆菌数量更高，30.7%的患者携带PPAD-T2变体。临床CP参数不受PPAD变体影响。然而，PPAD-T2影响口腔微生物群组成，使某些菌属富集。
虽然PPAD变体不影响CP严重程度，但它影响口腔微生物群组成。需要进一步研究以了解瓜氨酸化在口腔微生物群和慢性炎症性疾病发展中的作用。
