Csuzdi Csaba, Koo Jachoon, Hong Yong
Kenderesi str. 39, Piliscsaba, Hungary.
Division of Science Education and Institute of Fusion Science, College of Education, Jeonbuk National University, Jeonju 54896, Republic of Korea.
Zookeys. 2025 Mar 13;1231:293-310. doi: 10.3897/zookeys.1231.144623. eCollection 2025.
We present for the first time the complete mitochondrial genomes (mt genomes) of the earthworms and (Clitellata, Megadrili) collected in Hungary and Korea, respectively. The complete mt genome of comprised 15,014 base pairs. Lengths of the three complete mt genomes varied between 15,090 and 15,123 bp. All four mt genomes contained 13 protein-coding genes (PCGs), two rRNA genes, 22 tRNA genes, and one major non-coding control region. These mt genome arrangements are identical to those observed in the mt genomes of most earthworms, and all the 37 genes are transcribed from the same directional strand. All 13 PCGs had the same ATG start codon. Most of the PCGs end with TAA or TAG, whereas the remaining end with an incomplete stop codon, T. Stop codons were consistent in the PCGs throughout the mt genomes, except 5, which contains a TAG stop codon in ND5 instead of the TAA found in the other samples. Both species' genomes showed biased base composition, with 63.5% AT and 36.4% GC content in and 62.8% and 37.2% in . Phylogenetic analysis of the mt genomes corroborated the monophyly of the family Lumbricidae and the close relationship between and species pairs. The available sequences were embedded between the samples, thereby supporting the synonymy of the two names.
我们首次分别展示了在匈牙利和韩国采集的蚯蚓（环带纲，巨蚓科）的完整线粒体基因组（mt基因组）。蚯蚓的完整mt基因组由15,014个碱基对组成。三个完整的蚯蚓mt基因组长度在15,090至15,123碱基对之间变化。所有四个mt基因组都包含13个蛋白质编码基因（PCG）、两个rRNA基因、22个tRNA基因和一个主要的非编码控制区。这些mt基因组排列与大多数蚯蚓mt基因组中观察到的排列相同，并且所有37个基因都从同一方向链转录。所有13个PCG都有相同的ATG起始密码子。大多数PCG以TAA或TAG结尾，而其余的以不完全终止密码子T结尾。整个mt基因组中PCG的终止密码子是一致的，除了蚯蚓5，其ND5中含有TAG终止密码子，而不是其他样本中发现的TAA。两个物种的基因组都显示出碱基组成偏向，蚯蚓1的AT含量为63.5%，GC含量为36.4%，蚯蚓2的AT含量为62.8%，GC含量为37.2%。mt基因组的系统发育分析证实了正蚓科的单系性以及蚯蚓1和蚯蚓2物种对之间的密切关系。可用的序列嵌入在蚯蚓1和蚯蚓2样本之间，从而支持了这两个名称同义。
