Tong Yao, Wu Lian, Lin Yi-Jie, Ayivi Sam Pedro Galilee, Storey Kenneth B, Zhang Jia-Yong, Yu Dan-Na
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, Zhejiang Province, China.
Department of Biology, Carleton University, Ottawa, Canada.
Mitochondrial DNA B Resour. 2022 Jun 20;7(6):1093-1095. doi: 10.1080/23802359.2022.2086498. eCollection 2022.
The phylogenetic relationship of Ephemeridae (Insect: Ephemeroptera) remains hotly debated using mitochondrial (mt) genomes. All previously reported mt genomes of Ephemeridae belong to the genus . This study provides the first complete mt genome sequence from the genus with an analysis of the mitogenome of Mc Dunnough, 1924 (Ephemeroptera: Ephemeridae) and providing new information to discuss the phylogenetic relationships within Ephemeroptera. The complete mt genome of was a circular molecule of 16,159 bp in length, containing 37 genes (2 rRNA genes, 13 protein-coding genes, 22 tRNA genes), which showed the typical mt gene arrangement of insects. The AT content of the whole genome was 70.0% and the length of the control region was 1091 bp. All protein-coding genes used ATN as the start codon, and most PCGs used TAA/TAG as the stop codons excluding COI, COII, ND5 and Cyt b that used T as the stop codon. BI and ML phylogenetic trees constructed from 27 species of 13 families showed that Ephemeridae is a sister clade to the clade Polymitarcyidae.
利用线粒体（mt）基因组对蜉蝣科（昆虫纲：蜉蝣目）的系统发育关系仍存在激烈争论。此前报道的所有蜉蝣科线粒体基因组均属于 属。本研究提供了 属的首个完整线粒体基因组序列，对 1924 年麦克多诺的 （蜉蝣目：蜉蝣科）的线粒体基因组进行了分析，并为讨论蜉蝣目内部的系统发育关系提供了新信息。 的完整线粒体基因组是一个长度为 16,159 bp 的环状分子，包含 37 个基因（2 个 rRNA 基因、13 个蛋白质编码基因、22 个 tRNA 基因），呈现出昆虫典型的线粒体基因排列。全基因组的 AT 含量为 70.0%，控制区长度为 1091 bp。所有蛋白质编码基因均以 ATN 作为起始密码子，除了 COI、COII、ND5 和 Cyt b 以 T 作为终止密码子外，大多数蛋白质编码基因以 TAA/TAG 作为终止密码子。由 13 个科的 27 个物种构建的贝叶斯（BI）和最大似然（ML）系统发育树表明，蜉蝣科是多节蜉科分支的姐妹分支。
