Xiong Li, Li Qinxuan, Zhou Xuhui, Xiao Jiujia, Yang Xingyu, Xu Hengxiang, Guo Chuan
Clinical Medicine, Chengdu Medical College, Chengdu, China.
Department of Cardiology, The Second Affiliated Hospital of Soochow University, Suzhou, China.
J Alzheimers Dis Rep. 2025 Mar 21;9:25424823241312970. doi: 10.1177/25424823241312970. eCollection 2025 Jan-Dec.
Alzheimer's disease (AD) is a chronic neurodegenerative disease and the most prevalent form of dementia. Fenugreek seeds possess anti-inflammatory and antioxidant effects, making them valuable therapeutic agents in managing neurodegenerative diseases.
This study aimed to investigate the primary biological pathways and specific mechanisms underlying the protective effects of fenugreek in preventing mice of AD by employing bioinformatics and experimental verification.
We administered fenugreek extract as an intervention in mice model of AD and then assessed their cognitive ability and histopathological changes. We predicted the key target genes associated with fenugreek action on AD and the main biological pathways using the bioinformatics method. Furthermore, we observed the different expression of target genes by western blot (WB).
The bioinformatics analysis revealed a strong correlation between fenugreek and AD. The behavioral experiments confirmed that fenugreek improved the behavioral and cognitive dysfunction in mice with AD. The histopathology revealed significant changes that fenugreek can inhabit Nissl bodies. Western blot experiments confirmed that fenugreek exerted statistically significant modulatory effects on the levels of inflammatory proteins [interleukin-6 (IL-6), IL-10, and IL-1β] and oxidative stress-related proteins (amyloid-β protein precursor, apolipoprotein E, and presenilin 1).
This study suggested that fenugreek might be involved in the AD pathway and effectively prevented the progression of AD through significant anti-inflammatory and antioxidant effects.
阿尔茨海默病（AD）是一种慢性神经退行性疾病，也是最常见的痴呆形式。胡芦巴种子具有抗炎和抗氧化作用，使其成为治疗神经退行性疾病的重要治疗药物。
本研究旨在通过生物信息学和实验验证，探讨胡芦巴预防AD小鼠的主要生物学途径和具体机制。
我们将胡芦巴提取物作为干预措施应用于AD小鼠模型，然后评估其认知能力和组织病理学变化。我们使用生物信息学方法预测与胡芦巴对AD作用相关的关键靶基因和主要生物学途径。此外，我们通过蛋白质免疫印迹法（WB）观察靶基因的差异表达。
生物信息学分析显示胡芦巴与AD之间存在密切相关性。行为实验证实，胡芦巴改善了AD小鼠的行为和认知功能障碍。组织病理学显示，胡芦巴可使尼氏体发生显著变化。蛋白质免疫印迹实验证实，胡芦巴对炎症蛋白[白细胞介素-6（IL-6）、IL-10和IL-1β]和氧化应激相关蛋白（淀粉样β蛋白前体、载脂蛋白E和早老素1）的水平具有统计学意义的调节作用。
本研究表明，胡芦巴可能参与AD途径，并通过显著的抗炎和抗氧化作用有效预防AD的进展。
