Wang Qi, Zhang Wenwen, Liu Jiaqin, Qin Wenjun, Cai Jin
School of Life Science, Shanxi University, Taiyuan, 030006, Shanxi, China.
Inner Mongolia Yili Industrial Group Co., Ltd., Hohhot, 010110, Inner Mongolia, China.
Curr Microbiol. 2025 Mar 24;82(5):204. doi: 10.1007/s00284-025-04190-5.
Ulcerative colitis (UC) is a typical type of inflammatory bowel disease, which is often recurrent and directly related with colorectal cancer. Therefore, early prevention and treatment for UC is very necessary. Therefore, it is necessary to find efficient substances to treat the UC with less side effects than drugs. Exopolysaccharides (EPSs) are important bioactive constituents of lactic acid bacteria. The study evaluated the effects of EPS1 produced by Levilactobacillus brevis M-10 on UC by determining the weight, the disease activity index (DAI) and the physiological and biochemical indexes. The pathological structures of colon were observed. The gut microbiota and the short-chain fatty acids were analyzed. The results demonstrated high dose (HD) of EPS1 (400 mg/kg-BW) that had the best effects on UC mice. The HD group restored body weight, decreased DAI and alleviated shortening of the length of the colon, recovered liver tissue, declined lipopolysaccharide, and myeloperoxidase. Also the HD group showed that the expression of tight junction proteins increased, IL-10 up-regulated, IL-6, IL-1β, and TNF-α down-regulated, and the gut microbiota dysbiosis balanced. The HD group markedly elevated the relative abundance of Lachnospiraceae_NK4A136_group, Unclassified-Lachnospiraceae, and Unclassified- Muribaculaceae. Acetic acid, propionic acid, and n-butyric acid were significantly increased in the HD group (P < 0.05). The study could provide a theoretical basis and material support for the exploration of safe functional food in alleviating and preventing UC.
溃疡性结肠炎（UC）是一种典型的炎症性肠病，常反复发作且与结直肠癌直接相关。因此，对UC进行早期预防和治疗非常必要。因此，有必要寻找比药物副作用更小的有效物质来治疗UC。胞外多糖（EPSs）是乳酸菌的重要生物活性成分。本研究通过测定体重、疾病活动指数（DAI）以及生理生化指标，评估了短乳杆菌M-10产生的EPS1对UC的影响。观察了结肠的病理结构。分析了肠道微生物群和短链脂肪酸。结果表明，高剂量（HD）的EPS1（400mg/kg体重）对UC小鼠效果最佳。HD组恢复了体重，降低了DAI，减轻了结肠长度缩短，恢复了肝组织，降低了脂多糖和髓过氧化物酶。此外，HD组显示紧密连接蛋白表达增加，IL-10上调，IL-6、IL-1β和TNF-α下调，肠道微生物群失调得到平衡。HD组显著提高了毛螺菌科_NK4A136_组、未分类的毛螺菌科和未分类的Muribaculaceae的相对丰度。HD组乙酸、丙酸和正丁酸显著增加（P<0.05）。该研究可为探索缓解和预防UC的安全功能性食品提供理论依据和物质支持。
