Höfert Lisa, Franz Benjamin, Groß Cedric, Kuntze Delen, Jurásek Bronislav, Kuchař Martin, Dreßler Jan, Becker Susen, Baumann Sven
Institute of Forensic Medicine, Medical Faculty, University of Leipzig, Leipzig, Germany.
Forensic Laboratory of Biologically Active Substances, Department of Chemistry of Natural Compounds, University of Chemistry and Technology Prague, Prague, Czech Republic.
Sci Rep. 2025 Mar 24;15(1):10086. doi: 10.1038/s41598-025-93931-4.
The semi-synthetic cannabinoid hexahydrocannabinol (HHC) has become a highly discussed topic in forensic toxicology since 2022 due to its legal availability at this time and its psychoactive effects. This study aimed to investigate the pharmacokinetics, effects, and immunological detectability of HHC after oral (25 mg HHC fruit gum) and inhalative (three puffs from HHC vape) consumption with three participants per group. Serum (up to 48 h), urine (up to five days), and saliva (up to 48 h) samples were collected at different relevant time points and analyzed by HPLC-MS/MS for (9R)/(9S)-HHC, 11-hydroxy-HHC, and (9R)/(9S)-HHC carboxylic acid with a fully validated method. Additionally, immunological detectability was investigated with three different commercially available tests. To address the psychoactive effects, the subjective "high" feeling (scale 0-10) was monitored and different psychophysical tests (e.g. modified Romberg test, walk and turn) were conducted. Overall, the pharmacokinetics and effects of HHC were comparable to tetrahydrocannabinol (THC). However, the route of administration as well as inter-individual factors played a crucial role regarding maximum concentrations, pharmacokinetic profiles, and psychoactive effects.
自2022年以来，半合成大麻素六氢大麻酚（HHC）因其在当时的合法可得性及其精神活性作用，已成为法医毒理学中一个备受讨论的话题。本研究旨在调查口服（25毫克HHC水果口香糖）和吸入（从HHC电子烟吸三口）后，每组三名参与者体内HHC的药代动力学、效果和免疫检测性。在不同的相关时间点采集血清（长达48小时）、尿液（长达五天）和唾液（长达48小时）样本，并采用经过充分验证的方法，通过高效液相色谱-串联质谱法对（9R）/（9S）-HHC、11-羟基-HHC和（9R）/（9S）-HHC羧酸进行分析。此外，还使用三种不同的市售检测方法研究了免疫检测性。为了研究精神活性作用，监测了主观的“兴奋”感觉（0-10分），并进行了不同的心理物理学测试（如改良罗姆伯格测试、步行和转身测试）。总体而言，HHC的药代动力学和效果与四氢大麻酚（THC）相当。然而，给药途径以及个体间因素在最大浓度、药代动力学特征和精神活性作用方面起着关键作用。
