Zhang Yanan, Wang Yimin, Huang Ningjing, Zheng Wei, Guo Hang, Li Chunyin
College of Chemistry and Chemical Engineering, Huanggang Normal University, Huanggang, 438000, China.
College of Biology and Agricultural Resources, Huanggang Normal University, Huanggang, 438000, China.
Anal Methods. 2025 Apr 3;17(14):2894-2901. doi: 10.1039/d5ay00038f.
A sensor for non-enzymatic hydrogen peroxide (HO) detection was developed based on silicon quantum dots (SiQDs). The fluorescent SiQDs were synthesized using a one-pot hydrothermal method. The SiQDs were made water-soluble and pH-stable by covering their surfaces with amino and hydroxyl groups. Additionally, the prepared SiQDs had a large Stokes shift (131 nm), excellent photostability (442 min), and hypotoxicity (, 99% cell viability was retained after a 24 h-treatment at 8.0 mg mL of SiQDs). The dynamic quenching effect induced by HO effectively suppresses the blue fluorescence of SiQDs. Under the optimum conditions, the SiQD-based HO sensor exhibited a wide linear range (10-1000 μM), high sensitivity and selectivity, and good anti-interference ability. The detection limit and relative standard deviation were 1.7 μM and 4.5% ( = 20 μM, = 7), respectively. Accordingly, a simple, enzyme-free, and highly efficient method was established for detecting HO in aqueous solutions. Moreover, this eco-friendly fluorescent sensor successfully determined the HO concentration in human serum samples with 92.0-102.4% recoveries.
基于硅量子点（SiQDs）开发了一种用于非酶促过氧化氢（HO）检测的传感器。采用一锅水热法合成了荧光硅量子点。通过用氨基和羟基覆盖硅量子点的表面，使其具有水溶性和pH稳定性。此外，制备的硅量子点具有较大的斯托克斯位移（131 nm）、优异的光稳定性（442分钟）和低毒性（在8.0 mg/mL硅量子点中处理24小时后，细胞活力保留率达99%）。HO诱导的动态猝灭效应有效地抑制了硅量子点的蓝色荧光。在最佳条件下，基于硅量子点的HO传感器表现出宽线性范围（10 - 1000 μM）、高灵敏度和选择性以及良好的抗干扰能力。检测限和相对标准偏差分别为1.7 μM和4.5%（ = 20 μM， = 7）。因此，建立了一种简单、无酶且高效的水溶液中HO检测方法。此外，这种环保型荧光传感器成功测定了人血清样品中的HO浓度，回收率为92.0 - 10
