Gogoi Barbi, Phukon Pinkee, Dutta Sima Kumari, Das Angshurekha, Borkakoty Uttara, Barua Purnima
Viral Research Diagnostic Laboratory, Department of Microbiology, Jorhat Medical College & Hospital, Jorhat, Assam, India.
Department of Microbiology, Tinsukia Medical College & Hospital, Tinsukia, Assam, India.
Acta Trop. 2025 Apr;264:107588. doi: 10.1016/j.actatropica.2025.107588. Epub 2025 Mar 23.
Dengue is a significant mosquito-borne infection that causes public health concern, particularly in tropical and subtropical regions. This study aimed to characterize the molecular typing of dengue viruses in Jorhat district, as information on dengue serotypes is scarce in India.
A total of 1359 serum samples of clinically suspected dengue cases were tested for non-structural protein 1 (NS1) antigen and Immunoglobulin M (IgM) antibody using enzyme-linked immunosorbent assay (ELISA). Additionally, viral RNA was extracted from positive samples for multiplex reverse transcriptase-polymerase chain reaction (RT-PCR) to determine the dengue serotypes. Seasonal variations, gender, and age-wise incidences were also studied. Statistical analysis was performed to assess demographic and clinical characteristics.
This study depicts a substantial burden of dengue, with 370 (27.22 %) positive samples. IgM and NS1 ELISA tested positive for 42 (11.3 %) and 328 (88.6 %) samples. Dengue virus (DENV) cases predominantly affected males (58.4 %) and the age group of 16-30 years. The major clinical manifestations include fever, headache, and body aches. Serotyping revealed DENV-2 as the predominant serotype, followed by DENV-3 and DENV-1. The co-infection of DENV-2 and DENV-3 was observed in many cases. Seasonal clustering of dengue cases indicates the highest peak in September and October.
This study provides valuable insights into the epidemiology, diagnosis, and serotyping of dengue viruses in the Jorhat district of Assam, emphasizing the need for continued surveillance and effective vector control measures that should be implemented to mitigate the early detection of dengue in this region.
登革热是一种由蚊子传播的重要感染病，引起了公众对健康的关注，特别是在热带和亚热带地区。本研究旨在对乔哈特地区的登革热病毒进行分子分型，因为印度关于登革热血清型的信息匮乏。
使用酶联免疫吸附测定（ELISA）对1359份临床疑似登革热病例的血清样本进行非结构蛋白1（NS1）抗原和免疫球蛋白M（IgM）抗体检测。此外，从阳性样本中提取病毒RNA，用于多重逆转录聚合酶链反应（RT-PCR）以确定登革热血清型。还研究了季节性变化、性别和年龄发病率。进行统计分析以评估人口统计学和临床特征。
本研究表明登革热负担沉重，有370份（27.22%）阳性样本。IgM和NS1 ELISA检测分别有42份（11.3%）和328份（88.6%）样本呈阳性。登革热病毒（DENV）病例主要影响男性（58.4%）和16至30岁年龄组。主要临床表现包括发热、头痛和身体疼痛。血清分型显示DENV-2是主要血清型，其次是DENV-3和DENV-1。许多病例中观察到DENV-2和DENV-3的共同感染。登革热病例的季节性聚集表明9月和10月为最高峰。
本研究为阿萨姆邦乔哈特地区登革热病毒的流行病学、诊断和血清分型提供了有价值的见解，强调需要持续监测并实施有效的病媒控制措施，以减轻该地区登革热的早期检测。
