Sharma Pratibha, Malhotra Bharti, Sharma Himanshu, Bhomia Neha, Deeba Farah, Kuldeep Anjana, Trivedi Khushbu, Tiwari Jitendra, Kaur Harmanmeet, Vijay Neetu
Department of Microbiology, Sawai Man Singh Medical College, Jaipur, Rajasthan, India.
Department of Health Research, Indian Council of Medical Research, New Delhi, India.
Indian J Med Res. 2024 Nov;160(5):479-488. doi: 10.25259/ijmr_1343_23.
Background & objectives Dengue virus causes frequent outbreaks and epidemics with high morbidity and mortality. It is important to monitor the trends of the dengue virus and its serotypes. We carried out the present work to study the prevalence of the dengue virus and its serotypes in clinically suspected cases of dengue in Rajasthan. Methods A total of 1,47,777 individuals reporting as pyrexia of unknown origin or clinically suspected of dengue infection were included in this study. The presence of dengue NS1 antigen and dengue IgM was tested by ELISA. Nucleic acid extraction and PCR was done for detection of dengue virus RNA. Serotyping of representative dengue PCR-positive samples was done by real time PCR. Results Of the 1,47,777 dengue suspected cases, 28092 (19.01%) were positive for dengue by NS1antigen or IgM ELISA. Year-wise percentage positivity from 2015 to 2022 was 30.42, 16.49, 17.81, 20.15, 20.50, 9.25, 24.55 and 15.16 per cent, respectively. Males of age >15 yr were found to be more commonly affected. The number of dengue cases was significantly higher during the post-monsoon period throughout the eight-year study period. All four dengue serotypes circulated during the study period. DENV-2 and DENV-3 were the predominant serotypes during 2015 to 2017, while DENV-1 and DENV-2 were predominant during 2018 to 2022. Interpretation & conclusion The findings of this study suggest that the dengue positivity in Rajasthan was the highest in post-monsoon season among adult males. The serotype prevalent varied from time to time and was helpful in understanding the changing epidemiology of DENV.
登革病毒频繁引发发病率和死亡率高的疫情与流行。监测登革病毒及其血清型的趋势很重要。我们开展本研究以了解拉贾斯坦邦临床疑似登革热病例中登革病毒及其血清型的流行情况。方法：本研究纳入了总共147777名报告为不明原因发热或临床疑似登革热感染的个体。通过酶联免疫吸附测定法（ELISA）检测登革热非结构蛋白1（NS1）抗原和登革热免疫球蛋白M（IgM）。进行核酸提取和聚合酶链反应（PCR）以检测登革病毒核糖核酸（RNA）。通过实时PCR对代表性登革热PCR阳性样本进行血清分型。结果：在147777例疑似登革热病例中，28092例（19.01%）通过NS1抗原或IgM ELISA检测为登革热阳性。2015年至2022年逐年的阳性率分别为30.42%、16.49%、17.81%、20.15%、20.50%、9.25%、24.55%和15.16%。发现年龄大于15岁的男性更常受到感染。在整个八年研究期间，季风后期的登革热病例数显著更高。在研究期间，所有四种登革热血清型均有传播。2015年至2017年期间，登革热病毒2型（DENV-2）和登革热病毒3型（DENV-3）为主要血清型，而2018年至2022年期间，DENV-1和DENV-2为主要血清型。解读与结论：本研究结果表明，拉贾斯坦邦成年男性中登革热阳性率在季风后期最高。流行的血清型随时间变化，有助于了解登革热病毒不断变化的流行病学情况。
