[通过微阵列技术研究细胞面积对单个成牙本质细胞极化和分化的影响]
[Effects of cell area on single odontoblast polarization and differentiation via microarray technology].
作者信息
Li Huen, Yu Nianzuo, Li Xiheng, Tang Xiaoduo, Sun Yalu, Si Chao, Zhang Junhu, Chang Bei
机构信息
Dept. of Pediatric Dentistry, Hospital of Stomatology, Jilin University, Changchun 130021, China.
Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun 130021, China.
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2025 Apr 1;43(2):183-189. doi: 10.7518/hxkq.2025.2024392.
OBJECTIVES
This study aimed to explore the impact of cell spreading area on odontoblast polarization and differentiation using micropatterned surfaces ge-nerated by photolithography.
METHODS
Micropatterned surfaces with differential adhesive properties were prepared using polyethylene glycol diacrylate (PEGDA)-ba-sed photolithography. Human dental pulp stem cells (hD-PSCs) were isolated into single cells and cultured on micropatterned surfaces with areas of 1 800, 2 700, and 3 600 μm. Immunofluorescence staining was used to observe cell morphology and analyze the relocating of the golgi apparatus and nucleus. Alkaline phosphatase staining was preformed to examine odontogenic differentiation.
RESULTS
The hDPSCs were successfully isolated and cultured on micropatterned surfaces mimicking the morphology of polarized odontoblasts. Phalloidin staining confirmed that the isolated hDPSCs successfully recapitulated the morphology of predesigned micropatterns. Immunofluorescence staining showed that the polarization and differentiation levels of the hDPSCs with a 3600 μm area were significantly higher than those with 1 800 and 2 700 μm areas (<0.05).
CONCLUSIONS
The polarization and differentiation of single hDPSCs increased with the cell areas on micropatterned surfaces.
目的
本研究旨在利用光刻技术制备的微图案表面，探讨细胞铺展面积对成牙本质细胞极化和分化的影响。
方法
采用基于聚乙二醇二丙烯酸酯（PEGDA）的光刻技术制备具有不同黏附特性的微图案表面。将人牙髓干细胞（hDPSCs）分离为单细胞，并培养在面积分别为1800、2700和3600μm的微图案表面上。采用免疫荧光染色观察细胞形态，并分析高尔基体和细胞核的重新定位。进行碱性磷酸酶染色以检测成牙本质分化。
结果
hDPSCs成功分离并培养在模拟极化成牙本质细胞形态的微图案表面上。鬼笔环肽染色证实分离的hDPSCs成功重现了预先设计的微图案形态。免疫荧光染色显示，面积为3600μm的hDPSCs的极化和分化水平显著高于面积为1800和2700μm的hDPSCs（P<0.05）。
结论
在微图案表面上，单个hDPSCs的极化和分化随细胞面积增加而增强。
Zotero 插件