Hu Dongmei, Wu Qing, Yang Yujun, Wang Yan, Li Yanhao, Chen Haixiang, Tang Liang, Mao Xiang, Wang Zhenyu
College of Biomedical Engineering, Chongqing Medical University, Chongqing, 400016, People's Republic of China.
State Key Laboratory of Ultrasound in Medicine and Engineering College of Biomedical Engineering, Chongqing Medical University, Chongqing, 400016, People's Republic of China.
Discov Nano. 2025 Mar 25;20(1):55. doi: 10.1186/s11671-025-04232-8.
Metal-based nanoenzymes with excellent biocompatibility and stable chemical properties are an effective antimicrobial agent against bacterial resistance due to their radical-mediated catalysis. In this work, due to the pH of most bacterial infection sites being close to neutral, targeting the problem of FeO@Ag difficulty in maintaining the catalytic activity of nanoenzymes in neutral environments, we prepare a novel multifunctional FeO@Ag@ indocyanine green/adenosine triphosphate peroxidase nanoenzymes for synergistic antibacterial activity. ICG (Indocyanine Green) and ATP (Adenosine triphosphate) are adsorbed on the surface of FeO@Ag through electrostatic adsorption to form its structure. The cell viability remained above 90%, indicating its good biocompatibility. By complexing ATP with nanoenzymes to participate in single electron transfer and binding with Fe (II), ATP promotes the sudden release of hydroxyl radical (·OH) from the system, successfully transferring FeO@Ag the peroxidase activity of nanoenzymes extends to neutral pH. By utilizing ICG as a photosensitizer and a sonosensitizer, under the combined treatment of near-infrared light and ultrasound, the photodynamic therapy (PDT)/photothermal therapy (PTT)/sonodynamic therapy (SDT) functions can be achieved, achieving multifunctional synergistic antibacterial effects. In a neutral environment, its bactericidal efficiency against Gram negative (Escherichia coli) and Gram positive (Staphylococcus aureus) is 99.9% and 99.7%, respectively, providing a new multi-mode synergistic antibacterial strategy for bacterial infections.
具有优异生物相容性和稳定化学性质的金属基纳米酶是一种有效的抗菌剂，可通过自由基介导的催化作用对抗细菌耐药性。在这项工作中，由于大多数细菌感染部位的pH值接近中性，针对FeO@Ag在中性环境中难以维持纳米酶催化活性的问题，我们制备了一种新型多功能FeO@Ag@吲哚菁绿/三磷酸腺苷过氧化物酶纳米酶用于协同抗菌活性。吲哚菁绿（ICG）和三磷酸腺苷（ATP）通过静电吸附吸附在FeO@Ag表面形成其结构。细胞活力保持在90%以上，表明其具有良好的生物相容性。通过将ATP与纳米酶络合以参与单电子转移并与Fe（II）结合，ATP促进系统中羟基自由基（·OH）的突然释放，成功地将FeO@Ag纳米酶的过氧化物酶活性扩展到中性pH值。通过利用ICG作为光敏剂和声敏剂，在近红外光和超声的联合处理下，可实现光动力疗法（PDT）/光热疗法（PTT）/声动力疗法（SDT）功能，实现多功能协同抗菌效果。在中性环境中，其对革兰氏阴性菌（大肠杆菌）和革兰氏阳性菌（金黄色葡萄球菌）的杀菌效率分别为99.9%和99.7%，为细菌感染提供了一种新的多模式协同抗菌策略。
