与来自美国西南部沙漠土壤的phiNIT1相关噬菌体的比较基因组学
Comparative Genomics of Phages Related to phiNIT1 from Desert Soils of the Southwest United States.
作者信息
Vill Albert C, Delesalle Véronique A, Magness Leigh H, Chaudhry Brianne E, Lichty Katherine B, Strine Madison S, Guffey Alexandra A, DeCurzio Jenna M, Krukonis Greg P
机构信息
Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut, USA.
Department of Biology, Gettysburg College, Gettysburg, Pennsylvania, USA.
出版信息
Phage (New Rochelle). 2023 Dec 14;4(4):173-180. doi: 10.1089/phage.2023.0027. eCollection 2023 Dec.
BACKGROUND
is a Gram-positive bacterium commonly found in soil. Phages infecting have diverse genomes and may be used in therapeutics against pathogenic species.
MATERIALS AND METHODS
Phages were isolated and purified on . Comparative genomics was performed to determine taxonomy, a liquid-culture lysis assay was used to determine host range, and virion structure was observed for a representative phage by transmission electron microscopy.
RESULTS
Five phages were found to have high sequence similarity to each other and known phages, including phiNIT1. Annotation shows a conserved genome structure and protein families unique to this group. Repeated sequences are concentrated in intergenic regions at the ends of the genomes. Capsid structure and tail length suggest these phages are myoviruses.
CONCLUSIONS
The phages described here share sequence similarity and genomic structure with known phages. This study expands our understanding of the genetic diversity of phages.
背景
[细菌名称]是一种常见于土壤中的革兰氏阳性菌。感染[细菌名称]的噬菌体具有多样的基因组，可用于针对致病性[细菌名称]物种的治疗。
材料与方法
在[培养基名称]上分离并纯化噬菌体。进行比较基因组学以确定分类学，使用液体培养裂解试验确定宿主范围，并通过透射电子显微镜观察代表性噬菌体的病毒体结构。
结果
发现五个噬菌体彼此之间以及与已知噬菌体（包括phiNIT1）具有高度的序列相似性。注释显示该组具有保守的基因组结构和独特的蛋白质家族。重复序列集中在基因组末端的基因间区域。衣壳结构和尾长表明这些噬菌体是肌病毒。
结论
此处描述的噬菌体与已知的[细菌名称]噬菌体具有序列相似性和基因组结构。这项研究扩展了我们对[细菌名称]噬菌体遗传多样性的理解。
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