与来自美国西南部沙漠土壤的SIOphi相关的噬菌体。
Phages Related to SIOphi from Desert Soils of the Southwest United States.
作者信息
Magness Leigh H, Delesalle Véronique A, Vill Albert C, Strine Madison S, Chaudhry Brianne E, Lichty Katherine B, Guffey Alexandra A, DeCurzio Jenna M, Krukonis Greg P
机构信息
Department of Biology, Gettysburg College, Gettysburg, Pennsylvania, USA.
Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut, USA.
出版信息
Phage (New Rochelle). 2023 Dec 14;4(4):165-172. doi: 10.1089/phage.2023.0021. eCollection 2023 Dec.
BACKGROUND
Phages impact microbial dynamics, but our understanding of the diversity of phages that can lyse this model organism is limited.
MATERIALS AND METHODS
Phages were isolated from soil collected from two Southwest United States desert sites on wild strains. Phage genomes were assembled and bioinformatically characterized, and phage host range was assessed.
RESULTS
Four myoviruses with high nucleotide and amino acid similarity to each other (>97%) and to three phages in GenBank (>82%), including SIOphi, were isolated. These phages have double-stranded DNA genomes (153,882-156,577 bp), low GC content, and 256-270 putative protein coding genes (28-30% with predicted functions). Comparative genomics revealed differences in putative lysis and replication genes.
CONCLUSIONS
Comparative approaches provide insight into phage evolution, identifying unique genes shared within phage clusters. Better characterization of phages will aid in linking genetic differences among phages (e.g., lysin genes) to phenotype (e.g., host range).
背景
噬菌体影响微生物动态，但我们对能够裂解这种模式生物的噬菌体多样性的了解有限。
材料与方法
从美国西南部两个沙漠地点采集的土壤中分离出针对野生菌株的噬菌体。对噬菌体基因组进行组装和生物信息学表征，并评估噬菌体宿主范围。
结果
分离出了四种肌病毒，它们彼此之间以及与GenBank中的三种噬菌体（包括SIOphi）具有高度的核苷酸和氨基酸相似性（>97%）。这些噬菌体具有双链DNA基因组（153,882 - 156,577 bp），GC含量低，以及256 - 270个推定的蛋白质编码基因（28 - 30%具有预测功能）。比较基因组学揭示了推定的裂解和复制基因的差异。
结论
比较方法有助于深入了解噬菌体进化，识别噬菌体簇内共享的独特基因。更好地表征噬菌体将有助于将噬菌体之间的遗传差异（例如溶菌酶基因）与表型（例如宿主范围）联系起来。
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