Department of Integrated Science and Technology, James Madison University, Harrisonburg, VA 22807, USA.
Virology. 2013 Sep;444(1-2):374-83. doi: 10.1016/j.virol.2013.07.004. Epub 2013 Jul 30.
Twenty-eight bacteriophages infecting the local host Bacillus pumilus BL-8 were isolated, purified, and characterized. Nine genomes were sequenced, of which six were annotated and are the first of this host submitted to the public record. The 28 phages were divided into two groups by sequence and morphological similarity, yielding 27 cluster BpA phages and 1 cluster BpB phage, which is a BL-8 prophage. Most of the BpA phages have a host range restricted to distantly related strains, B. pumilus and B. simplex, reflecting the complexities of Bacillus taxonomy. Despite isolation over wide geographic and temporal space, the six cluster BpA phages share most of their 23 functionally annotated protein features and show a high degree of sequence similarity, which is unique among phages of the Bacillus genera. This is the first report of B. pumilus phages since 1981.
从当地宿主解淀粉芽孢杆菌 BL-8 中分离、纯化并鉴定了 28 株噬菌体。对其中 9 个基因组进行了测序，其中 6 个得到了注释，这是该宿主首次提交给公众记录。根据序列和形态相似性，28 个噬菌体被分为两组，产生了 27 个 BpA 噬菌体群和 1 个 BpB 噬菌体群，后者是 BL-8 的前噬菌体。大多数 BpA 噬菌体的宿主范围仅限于亲缘关系较远的菌株，即解淀粉芽孢杆菌和短小芽孢杆菌，这反映了芽孢杆菌分类的复杂性。尽管在广泛的地理和时间空间中进行了分离，但六个 BpA 噬菌体群共享其 23 个具有功能注释的蛋白质特征的大部分，并显示出高度的序列相似性，这在芽孢杆菌属的噬菌体中是独一无二的。这是自 1981 年以来首次报道解淀粉芽孢杆菌噬菌体。
