Huang Guoqing, Wang Dandan, Xue Jiaying
Institute of Advanced Technology, Heilongjiang Academy of Sciences, Harbin 150001, China.
Curr Issues Mol Biol. 2025 Feb 26;47(3):150. doi: 10.3390/cimb47030150.
Alternative splicing (AS) plays a crucial role in regulating gene expression and protein diversity, influencing both normal cellular function and pathological conditions, including cancer. Protein pre-mRNA processing factor 8 (PRPF8), a core component of the spliceosome, is integral to the splicing process, ensuring accurate gene transcription and spliceosome assembly. Disruptions in PRPF8 function are linked to a variety of cancers, as mutations in this gene can induce abnormal splicing events that contribute to tumorigenesis, metastasis, and drug resistance. This review provides an in-depth analysis of the mechanisms by which PRPF8 regulates tumorigenesis through AS, exploring its role in diverse cancer types, including breast, liver, myeloid, and colorectal cancers. Furthermore, we examine the molecular pathways associated with PRPF8 dysregulation and their impact on cancer progression. We also discuss the emerging potential of targeting PRPF8 in cancer therapy, highlighting challenges in drug development.
可变剪接（Alternative splicing，AS）在调节基因表达和蛋白质多样性方面发挥着关键作用，影响正常细胞功能和包括癌症在内的病理状况。蛋白质前体mRNA加工因子8（PRPF8）是剪接体的核心组成部分，对剪接过程至关重要，可确保准确的基因转录和剪接体组装。PRPF8功能的破坏与多种癌症相关，因为该基因的突变可诱导异常剪接事件，从而促进肿瘤发生、转移和耐药性。本综述深入分析了PRPF8通过可变剪接调节肿瘤发生的机制，探讨其在多种癌症类型中的作用，包括乳腺癌、肝癌、髓系癌和结直肠癌。此外，我们研究了与PRPF8失调相关的分子途径及其对癌症进展的影响。我们还讨论了在癌症治疗中靶向PRPF8的新兴潜力，强调了药物开发中的挑战。
