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剪接因子 PRPF8 在乳腺癌中的作用。

The role of splicing factor PRPF8 in breast cancer.

机构信息

Institute of Advanced Technology, Heilongjiang Academy of Sciences, Harbin, Heilongjiang, China.

Institute of Cancer Prevention and Treatment, Heilongjiang Province Academy of Medical Sciences, Harbin, Heilongjiang, China.

出版信息

Technol Health Care. 2022;30(S1):293-301. doi: 10.3233/THC-THC228028.

DOI:10.3233/THC-THC228028
PMID:35124606
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9051661/
Abstract

BACKGROUND

Alternative splicing is a mechanism to produce different proteins with diverse functions from one gene. Many splicing factors play an important role in cancer progression. PRPF8 is a core protein component of the spliceosome complex, U4/U6-U5 tri-snRNP.

OBJECTIVE

However, PRPF8 involved in mRNA alternative splicing are rarely included in the prognosis.

METHODS

We found that PRPF8 was expressed in all examined cancer types. Further analyses found that PRPF8 expression was significantly different between the breast cancer and paracancerous tissues.

RESULTS

Survival analyses showed that PRPF8-high patients had a poor prognosis, and the expression of PRPF8 is associated with distant metastasis-free survival (DMFS) and post progression survival (PPS). Gene Set Enrichment Analysis (GSEA) has revealed that PRPF8 expression is correlated with TGF-β, JAK-STAT, and cell cycle control pathways. Consistent with these results, upon PRPF8 silencing, the growth of MCF-7 cells was reduced, the ability of cell clone formation was weakened, and p⁢21 expression was increased.

CONCLUSIONS

These results have revealed that PRPF8 is a significant factor for splicing in breast cancer progression.

摘要

背景

可变剪接是一种从一个基因产生具有不同功能的多种蛋白质的机制。许多剪接因子在癌症进展中发挥着重要作用。PRPF8 是剪接体复合物 U4/U6-U5 三 snRNP 的核心蛋白组成部分。

目的

然而，参与 mRNA 可变剪接的 PRPF8 很少被包含在预后中。

方法

我们发现 PRPF8 在所有检查的癌症类型中均有表达。进一步的分析发现，PRPF8 在乳腺癌和癌旁组织之间的表达存在显著差异。

结果

生存分析表明 PRPF8 高表达的患者预后不良，PRPF8 的表达与无远处转移生存（DMFS）和进展后生存（PPS）相关。基因集富集分析（GSEA）表明 PRPF8 的表达与 TGF-β、JAK-STAT 和细胞周期控制途径相关。与这些结果一致的是，沉默 PRPF8 后，MCF-7 细胞的生长减少，细胞克隆形成能力减弱，p⁢21 的表达增加。

结论

这些结果表明 PRPF8 是乳腺癌进展中剪接的一个重要因素。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6535/9051661/19603558e158/thc-30-thc228028-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6535/9051661/90a3097f0fe7/thc-30-thc228028-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6535/9051661/41c3de8e4617/thc-30-thc228028-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6535/9051661/8104a9fe35b4/thc-30-thc228028-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6535/9051661/19603558e158/thc-30-thc228028-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6535/9051661/90a3097f0fe7/thc-30-thc228028-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6535/9051661/41c3de8e4617/thc-30-thc228028-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6535/9051661/8104a9fe35b4/thc-30-thc228028-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6535/9051661/19603558e158/thc-30-thc228028-g004.jpg

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Front Cell Dev Biol. 2020 Jun 12;8:474. doi: 10.3389/fcell.2020.00474. eCollection 2020.
2
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Gene. 2019 Jun 15;701:32-40. doi: 10.1016/j.gene.2019.02.082. Epub 2019 Mar 18.
3
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Int J Mol Sci. 2024 Dec 26;26(1):127. doi: 10.3390/ijms26010127.
4
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Cell Mol Life Sci. 2024 Dec 27;82(1):18. doi: 10.1007/s00018-024-05515-6.
5
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Cancers (Basel). 2024 Sep 26;16(19):3282. doi: 10.3390/cancers16193282.
6
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J Transl Med. 2023 Dec 4;21(1):879. doi: 10.1186/s12967-023-04754-8.
7
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Front Genet. 2022 Sep 20;13:949850. doi: 10.3389/fgene.2022.949850. eCollection 2022.
长链非编码 RNA-p21 在胸主动脉瘤中的上调通过激活 TGF-β1 信号通路参与血管平滑肌细胞的增殖和凋亡的调节。
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4
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Nat Biotechnol. 2018 Dec;36(11):1056-1058. doi: 10.1038/nbt.4239. Epub 2018 Aug 16.
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6
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Anticancer Agents Med Chem. 2018;18(2):210-215. doi: 10.2174/1871520617666170912133054.
7
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Mol Cancer Ther. 2017 Dec;16(12):2849-2861. doi: 10.1158/1535-7163.MCT-17-0461. Epub 2017 Sep 6.
8
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9
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10
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Nat Rev Mol Cell Biol. 2017 Jul;18(7):437-451. doi: 10.1038/nrm.2017.27. Epub 2017 May 10.