Pham Thao Ngoc, Vu Hoa Thi, Tasaki Takafumi, Pham-The Tai, Tran Nghi Ngoc, Nishijo Muneko, Tran Tien Viet, Tran Hai Anh, Takiguchi Tomoya, Nishino Yoshikazu
Department of Functional Diagnosis, Military Hospital 103, Vietnam Military Medical University, Hanoi 12108, Vietnam.
Department of Military Hygiene, Vietnam Military Medical University, Hanoi 12108, Vietnam.
Toxics. 2025 Mar 7;13(3):191. doi: 10.3390/toxics13030191.
We investigated the impact of perinatal dioxin exposure (indicated by dioxin levels in maternal breast milk) on clock gene mRNA expression in buccal cells of 9-year-old children from the Da Nang birth cohort in Vietnam using reverse transcription polymerase chain reaction. Of the 56 boys and 34 girls (67% detection rate) in whom was detected, was detected in only 16 boys and 15 girls. Dioxin levels were significantly higher in girls with detection than in girls without detection. In girls, higher relative expression levels were associated with greater levels of 2,3,7,8-tetrachlorodibenzo-p-dioxin and toxic equivalents of polychlorinated dibenzodioxins and polychlorinated dibenzofurans. Moreover, expression levels were correlated with shorter night sleep duration on weekdays, greater sleep duration on holidays, and higher hyperactivity scores. After adjusting for maternal parity, relative expression levels were higher in boys with higher toxic equivalents of polychlorinated dibenzofuran than those in girls. Although higher expression levels were correlated with greater verbal aggression and hostility scores in girls, no such associations were found in boys. These findings suggest the possible existence of sex-specific effects of perinatal dioxin exposure on circadian rhythms regulated by clock genes, particularly , leading to sleep and behavioral problems in later life.
我们利用逆转录聚合酶链反应，研究了围产期二噁英暴露（以母乳中的二噁英水平表示）对越南岘港出生队列中9岁儿童颊细胞中时钟基因mRNA表达的影响。在检测出二噁英的56名男孩和34名女孩（检测率67%）中，仅在16名男孩和15名女孩中检测出了[具体物质未明确]。检测出[具体物质未明确]的女孩中二噁英水平显著高于未检测出的女孩。在女孩中，相对[具体基因未明确]表达水平较高与2,3,7,8 - 四氯二苯并 - p - 二噁英以及多氯二苯并二噁英和多氯二苯并呋喃的毒性当量水平较高有关。此外，[具体基因未明确]表达水平与工作日较短的夜间睡眠时间、节假日较长的睡眠时间以及较高的多动评分相关。在调整产妇胎次后，多氯二苯并呋喃毒性当量较高的男孩中相对[具体基因未明确]表达水平高于女孩。虽然较高的[具体基因未明确]表达水平与女孩较高的言语攻击性和敌意评分相关，但在男孩中未发现此类关联。这些发现表明围产期二噁英暴露对由时钟基因（特别是[具体基因未明确]）调节的昼夜节律可能存在性别特异性影响，从而导致后期生活中的睡眠和行为问题。
