Hong Minh-Triet, Lee Giyoung, Chang Young-Tae
Department of Chemistry, Pohang University of Science and Technology, Pohang 37673, Republic of Korea.
Molecular Imaging Center, Pohang University of Science and Technology, Pohang 37673, Republic of Korea.
Tomography. 2025 Mar 14;11(3):34. doi: 10.3390/tomography11030034.
BACKGROUND/OBJECTIVES: Holotomography is an advanced imaging technique that enables high-resolution, three-dimensional visualization of microscopic specimens without the need for fixation or staining. Here we aim to apply holotomography technology to image live in their native state, avoiding invasive sample preparation procedures and phototoxic effects associated with other imaging modalities.
We use a low concentration of 7% ethanol for tardigrade sedation and sample preparation. Holotomographic images were obtained and reconstructed using the Tomocube HT-X1 system, enabling high-resolution visualization of tardigrade anatomical structures.
We captured detailed, label-free holotomography images of both external and internal structures of tardigrade, including the digestive tract, brain, ovary, claws, salivary glands, and musculature.
Our findings highlight holotomography as a complementary high-resolution imaging modality that effectively addresses the challenges faced with traditional imaging techniques in tardigrade research.
背景/目的：全息断层扫描是一种先进的成像技术，无需固定或染色就能对微观标本进行高分辨率的三维可视化。在此，我们旨在应用全息断层扫描技术对处于自然状态的活体进行成像，避免与其他成像方式相关的侵入性样本制备程序和光毒性效应。
我们使用低浓度的7%乙醇进行缓步动物镇静和样本制备。使用Tomocube HT-X1系统获取并重建全息断层扫描图像，从而实现对缓步动物解剖结构的高分辨率可视化。
我们获取了缓步动物外部和内部结构的详细、无标记全息断层扫描图像，包括消化道、大脑、卵巢、爪子、唾液腺和肌肉组织。
我们的研究结果凸显了全息断层扫描作为一种补充性高分辨率成像方式，能有效解决传统成像技术在缓步动物研究中面临的挑战。
