Balson Jordan, Boudreau Jeffrey R, Chin-Sang Ian D, Wang Yuxiang, Lefebvre Daniel D
Department of Biology, Queen's University, 116 Barrie St., Kingston, ON K7L 3J9, Canada.
Toxins (Basel). 2025 Feb 27;17(3):109. doi: 10.3390/toxins17030109.
Reported incidences of cyanobacterial harmful algal blooms (CHABs) are increasing across the world due to climate change and nutrient loading, dominating freshwater ecosystems and producing dangerous cyanotoxins that cause ecological damage. is one of the most common species of cyanobacteria; it produces hepatotoxic and neurotoxic microcystin-LR. The ecological and human impact of algal blooms is immense, and traditional CHAB remediation methods are not always adequate in eutrophic regions such as Lake Erie in North America. As a result, a proactive, targeted approach is needed to bioremediate cyanobacteria in their pre-colonial stages. Nematodes, such as the model organism , are potential candidates for bioremediating cyanobacteria such as . have metabolic pathways that could detoxify microcystin-LR and enable tolerance to cyanobacteria in nature. We analyzed health and fat accumulation on a diet of toxic and found that can ingest, digest, metabolize, and survive off of this diet. The mean lifespans of the worm populations were only slightly different at 20.68 ± 0.35 (mean ± S.E.M) and 17.89 ± 0.40 when fed and toxic , respectively. In addition, a diet of toxic compared to did not have any significant impact on pharyngeal pumping (304.2 ± 9.3 versus 330.0 ± 10.4 pumps/min), dauer response (86.3 ± 1.0 versus 83.65 ± 1.0% in dauer), mobility (209.25 ± 7.0 versus 210.15 ± 4.4 thrashes/min), or SKN-1 expression based on SKN1::GFP fluorescence measurements. Overall, a diet of toxic was able to sustain development, and was tolerant of it. These results suggest that and similar nematodes could be viable candidates for cyanobacterial bioremediation.
由于气候变化和养分负荷，世界各地报告的蓝藻有害藻华（CHABs）发生率正在上升，这些藻华在淡水生态系统中占主导地位，并产生危险的蓝藻毒素，造成生态破坏。微囊藻是最常见的蓝藻物种之一；它会产生具有肝毒性和神经毒性的微囊藻毒素-LR。藻华对生态和人类的影响是巨大的，而传统的CHAB修复方法在北美伊利湖等富营养化地区并不总是足够的。因此，需要一种积极主动、有针对性的方法来在蓝藻的前殖民阶段对其进行生物修复。线虫，如模式生物秀丽隐杆线虫，是对微囊藻等蓝藻进行生物修复的潜在候选者。秀丽隐杆线虫具有能够使微囊藻毒素-LR解毒并使其在自然环境中耐受蓝藻的代谢途径。我们分析了以有毒微囊藻为食时秀丽隐杆线虫的健康状况和脂肪积累情况，发现秀丽隐杆线虫能够摄取、消化、代谢这种食物并存活下来。当分别喂食微囊藻和有毒微囊藻时，线虫种群的平均寿命仅略有不同，分别为20.68±0.35（平均值±标准误）和17.89±0.40。此外，与喂食微囊藻相比，喂食有毒微囊藻对秀丽隐杆线虫的咽泵动（304.2±9.3次/分钟与330.0±10.4次/分钟）、滞育反应（滞育率为86.3±1.0%与83.65±1.0%）、活动能力（209.25±7.0次/分钟与210.15±4.4次/分钟）或基于SKN1::GFP荧光测量的SKN-1表达均无显著影响。总体而言，以有毒微囊藻为食能够维持秀丽隐杆线虫的发育，并且秀丽隐杆线虫能够耐受它。这些结果表明，秀丽隐杆线虫和类似的线虫可能是蓝藻生物修复的可行候选者。
