Liu Yilan, Benitez Mauricio Garcia, Chen Jinjin, Harrison Emma, Khusnutdinova Anna N, Mahadevan Radhakrishnan
Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada.
Institute of Biomedical Engineering, University of Toronto, Toronto, ON, Canada.
Front Bioeng Biotechnol. 2021 Jan 26;9:613322. doi: 10.3389/fbioe.2021.613322. eCollection 2021.
Global warming and uneven distribution of fossil fuels worldwide concerns have spurred the development of alternative, renewable, sustainable, and environmentally friendly resources. From an engineering perspective, biosynthesis of fatty acid-derived chemicals (FACs) is an attractive and promising solution to produce chemicals from abundant renewable feedstocks and carbon dioxide in microbial chassis. However, several factors limit the viability of this process. This review first summarizes the types of FACs and their widely applications. Next, we take a deep look into the microbial platform to produce FACs, give an outlook for the platform development. Then we discuss the bottlenecks in metabolic pathways and supply possible solutions correspondingly. Finally, we highlight the most recent advances in the fast-growing model-based strain design for FACs biosynthesis.
全球变暖和全球范围内化石燃料分布不均的问题促使人们开发替代、可再生、可持续且环境友好的资源。从工程学角度来看，脂肪酸衍生化学品（FACs）的生物合成是一种极具吸引力且前景广阔的解决方案，可利用微生物底盘中丰富的可再生原料和二氧化碳来生产化学品。然而，有几个因素限制了这一过程的可行性。本综述首先总结了FACs的类型及其广泛应用。接下来，我们深入研究用于生产FACs的微生物平台，并对该平台的发展进行展望。然后我们讨论代谢途径中的瓶颈并相应地提供可能的解决方案。最后，我们重点介绍在快速发展的基于模型的FACs生物合成菌株设计方面的最新进展。
