• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

巨噬细胞靶向的信使核糖核酸-脂质纳米颗粒促进小鼠创伤性脊髓损伤后的运动功能恢复。

Macrophage-targeted mRNA-lipid nanoparticles promote locomotor functional recovery after traumatic spinal cord injury in mice.

作者信息

Fu Chunyan, Jin Xiaoqin, Ji Kangfan, Lan Ke, Mao Xingjia, Huang Zhaobo, Chen Jian, Zhao Fengdong, Li Pengfei, Hu Xuefei, Sun Liwen, Lu Ning, Zhong Jinjie, Chen Yingying, Wang Linlin

机构信息

Department of Orthopaedics of Sir Run Run Shaw Hospital and Department of Basic Medicine Sciences, Zhejiang University School of Medicine, Hangzhou 310016, PR China.

Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, PR China.

出版信息

Sci Adv. 2025 Mar 28;11(13):eads2295. doi: 10.1126/sciadv.ads2295. Epub 2025 Mar 26.

DOI:10.1126/sciadv.ads2295
PMID:40138430
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11939073/
Abstract

Traumatic spinal cord injury (SCI) causes severe central nervous system damage. M2 macrophages within the lesion are crucial for SCI recovery. Our previous research revealed that M2 macrophages transfected with magnetotactic bacteria-derived gene can resist ferroptosis and enhance SCI recovery. To address the limitations of M2 macrophage transplantation, we developed lipid nanoparticles (LNPs) encapsulating mRNA targeting macrophages ( mRNA-PS/LNPs). The targeting efficiency and therapeutic effect of these LNPs in SCI mice were evaluated. Intravenous administration of mRNA-PS/LNPs delivered more mRNAs to lesion-site macrophages than those in the mRNA-LNP group, which resulted in enhancing motor function recovery, reducing lesion area and scar formation, and promoting neuronal survival and nerve fiber repair. These effects were nullified when macrophages were depleted. These findings suggest that macrophage-targeted delivery of mRNA is a promising therapeutic strategy for promoting spinal cord repair and motor function recovery in patients with traumatic SCI.

摘要

创伤性脊髓损伤（SCI）会导致严重的中枢神经系统损伤。损伤部位的M2巨噬细胞对SCI的恢复至关重要。我们之前的研究表明，转染了趋磁细菌衍生基因的M2巨噬细胞可以抵抗铁死亡并促进SCI恢复。为了解决M2巨噬细胞移植的局限性，我们开发了包裹靶向巨噬细胞的mRNA的脂质纳米颗粒（mRNA-PS/LNPs）。评估了这些LNPs在SCI小鼠中的靶向效率和治疗效果。静脉注射mRNA-PS/LNPs比mRNA-LNP组向损伤部位巨噬细胞递送了更多的mRNA，这导致运动功能恢复增强、损伤面积和瘢痕形成减少，并促进神经元存活和神经纤维修复。当巨噬细胞被清除时，这些作用消失。这些发现表明，巨噬细胞靶向递送mRNA是促进创伤性SCI患者脊髓修复和运动功能恢复的一种有前景的治疗策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c68/11939073/a45b458a19c0/sciadv.ads2295-f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c68/11939073/1d8c5fb00aef/sciadv.ads2295-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c68/11939073/5cdad0b346cf/sciadv.ads2295-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c68/11939073/6740beb52d4a/sciadv.ads2295-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c68/11939073/47b94945485c/sciadv.ads2295-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c68/11939073/8a071467bb8b/sciadv.ads2295-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c68/11939073/467d39c7d112/sciadv.ads2295-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c68/11939073/0bef427a6043/sciadv.ads2295-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c68/11939073/7c7f7872b85c/sciadv.ads2295-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c68/11939073/ad736fcbf22c/sciadv.ads2295-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c68/11939073/a45b458a19c0/sciadv.ads2295-f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c68/11939073/1d8c5fb00aef/sciadv.ads2295-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c68/11939073/5cdad0b346cf/sciadv.ads2295-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c68/11939073/6740beb52d4a/sciadv.ads2295-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c68/11939073/47b94945485c/sciadv.ads2295-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c68/11939073/8a071467bb8b/sciadv.ads2295-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c68/11939073/467d39c7d112/sciadv.ads2295-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c68/11939073/0bef427a6043/sciadv.ads2295-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c68/11939073/7c7f7872b85c/sciadv.ads2295-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c68/11939073/ad736fcbf22c/sciadv.ads2295-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c68/11939073/a45b458a19c0/sciadv.ads2295-f10.jpg

相似文献

1
Macrophage-targeted mRNA-lipid nanoparticles promote locomotor functional recovery after traumatic spinal cord injury in mice.巨噬细胞靶向的信使核糖核酸-脂质纳米颗粒促进小鼠创伤性脊髓损伤后的运动功能恢复。
Sci Adv. 2025 Mar 28;11(13):eads2295. doi: 10.1126/sciadv.ads2295. Epub 2025 Mar 26.
2
Methylprednisolone substituted lipid nanoparticles deliver C3 transferase mRNA for combined treatment of spinal cord injury.甲基强的松龙取代的脂质纳米颗粒递送C3转移酶mRNA用于脊髓损伤的联合治疗。
J Nanobiotechnology. 2025 Feb 8;23(1):98. doi: 10.1186/s12951-025-03153-z.
3
Macrophage Transcriptional Profile Identifies Lipid Catabolic Pathways That Can Be Therapeutically Targeted after Spinal Cord Injury.巨噬细胞转录谱鉴定出脊髓损伤后可作为治疗靶点的脂质分解代谢途径。
J Neurosci. 2017 Mar 1;37(9):2362-2376. doi: 10.1523/JNEUROSCI.2751-16.2017. Epub 2017 Jan 27.
4
Intravenous delivery of microRNA-133b along with Argonaute-2 enhances spinal cord recovery following cervical contusion in mice.静脉注射 microRNA-133b 联合 Argonaute-2 增强小鼠颈挫伤后脊髓的恢复。
Spine J. 2020 Jul;20(7):1138-1151. doi: 10.1016/j.spinee.2020.02.019. Epub 2020 Mar 4.
5
Rescuing macrophage normal function in spinal cord injury with embryonic stem cell conditioned media.用胚胎干细胞条件培养基挽救脊髓损伤中巨噬细胞的正常功能。
Mol Brain. 2016 May 6;9(1):48. doi: 10.1186/s13041-016-0233-3.
6
An Agonist of the Protective Factor SIRT1 Improves Functional Recovery and Promotes Neuronal Survival by Attenuating Inflammation after Spinal Cord Injury.保护性因子SIRT1的激动剂通过减轻脊髓损伤后的炎症反应来改善功能恢复并促进神经元存活。
J Neurosci. 2017 Mar 15;37(11):2916-2930. doi: 10.1523/JNEUROSCI.3046-16.2017. Epub 2017 Feb 13.
7
The immunomodulator decoy receptor 3 improves locomotor functional recovery after spinal cord injury.免疫调节剂诱饵受体3可改善脊髓损伤后的运动功能恢复。
J Neuroinflammation. 2016 Jun 17;13(1):154. doi: 10.1186/s12974-016-0623-6.
8
Age decreases macrophage IL-10 expression: Implications for functional recovery and tissue repair in spinal cord injury.年龄降低巨噬细胞白细胞介素-10的表达：对脊髓损伤后功能恢复和组织修复的影响。
Exp Neurol. 2015 Nov;273:83-91. doi: 10.1016/j.expneurol.2015.08.001. Epub 2015 Aug 8.
9
Transplantation of Human umbilical cord mesenchymal stem cells promotes functional recovery after spinal cord injury by blocking the expression of IL-7.人脐带间充质干细胞移植通过抑制 IL-7 的表达促进脊髓损伤后的功能恢复。
Eur Rev Med Pharmacol Sci. 2018 Oct;22(19):6436-6447. doi: 10.26355/eurrev_201810_16056.
10
Transplantation of miR-145a-5p modified M2 type microglia promotes the tissue repair of spinal cord injury in mice.miR-145a-5p 修饰的 M2 型小胶质细胞移植促进小鼠脊髓损伤组织修复。
J Transl Med. 2024 Aug 5;22(1):724. doi: 10.1186/s12967-024-05492-1.

引用本文的文献

1
Stepwise Administration of Bone-Targeted Lipid Nanoparticles Encapsulating Valproic Acid and TUDCA Facilitates In Vivo Direct Reprogramming for Osteoporosis Treatment.逐步给予包裹丙戊酸和牛磺熊去氧胆酸的骨靶向脂质纳米颗粒有助于体内直接重编程以治疗骨质疏松症。
Tissue Eng Regen Med. 2025 Jun 24. doi: 10.1007/s13770-025-00738-5.

本文引用的文献

1
Targeting lipid nanoparticles to the blood-brain barrier to ameliorate acute ischemic stroke.将脂质纳米颗粒靶向血脑屏障以改善急性缺血性中风。
Mol Ther. 2024 May 1;32(5):1344-1358. doi: 10.1016/j.ymthe.2024.03.004. Epub 2024 Mar 7.
2
Development of finely tuned liposome nanoplatform for macrophage depletion.开发用于耗竭巨噬细胞的精细调节脂质体纳米平台。
J Nanobiotechnology. 2024 Feb 29;22(1):83. doi: 10.1186/s12951-024-02325-7.
3
In Situ Piezoelectric-Catalytic Anti-Inflammation Promotes the Rehabilitation of Acute Spinal Cord Injury in Synergy.
原位压电催化抗炎协同促进急性脊髓损伤的修复
Adv Mater. 2024 May;36(18):e2311429. doi: 10.1002/adma.202311429. Epub 2024 Feb 8.
4
A new era of macrophage-based cell therapy.基于巨噬细胞的细胞治疗新时代。
Exp Mol Med. 2023 Sep;55(9):1945-1954. doi: 10.1038/s12276-023-01068-z. Epub 2023 Sep 1.
5
In vivo hematopoietic stem cell modification by mRNA delivery.mRNA 递送介导的体内造血干细胞修饰。
Science. 2023 Jul 28;381(6656):436-443. doi: 10.1126/science.ade6967. Epub 2023 Jul 27.
6
Porous microneedle patch with sustained delivery of extracellular vesicles mitigates severe spinal cord injury.多孔微针贴片持续递送细胞外囊泡减轻严重脊髓损伤。
Nat Commun. 2023 Jul 7;14(1):4011. doi: 10.1038/s41467-023-39745-2.
7
Spinal cord injury: molecular mechanisms and therapeutic interventions.脊髓损伤：分子机制与治疗干预。
Signal Transduct Target Ther. 2023 Jun 26;8(1):245. doi: 10.1038/s41392-023-01477-6.
8
Cooperative assembly of a designer peptide and silk fibroin into hybrid nanofiber gels for neural regeneration after spinal cord injury.设计肽与丝素蛋白协同组装成杂化纳米纤维凝胶，用于脊髓损伤后的神经再生。
Sci Adv. 2023 Jun 23;9(25):eadg0234. doi: 10.1126/sciadv.adg0234.
9
Cyclic helix B peptide alleviates proinflammatory cell death and improves functional recovery after traumatic spinal cord injury.环状螺旋 B 肽减轻促炎细胞死亡，改善创伤性脊髓损伤后的功能恢复。
Redox Biol. 2023 Aug;64:102767. doi: 10.1016/j.redox.2023.102767. Epub 2023 May 30.
10
Ionizable lipid nanoparticles deliver mRNA to pancreatic β cells via macrophage-mediated gene transfer.可离子化脂质纳米颗粒通过巨噬细胞介导的基因转移将 mRNA 递送至胰腺β细胞。
Sci Adv. 2023 Jan 27;9(4):eade1444. doi: 10.1126/sciadv.ade1444.