Pongprom Angkana, Bunkoed Opas
Center of Excellence for Innovation in Chemistry, Division of Physical Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.
Center of Excellence for Innovation in Chemistry, Division of Physical Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.
Talanta. 2025 Sep 1;292:128001. doi: 10.1016/j.talanta.2025.128001. Epub 2025 Mar 21.
A fluorescent probe was successfully developed using nitrogen-doped graphene quantum dots (N-GQDs) decorated porous zinc oxide (ZnO) embedded in a molecularly imprinted polymer (MIP). The ZnO∗N-GQDs∗MIP sensing probe was synthesized via the sol-gel polymerization method and exhibited a strong fluorescence emission at 403 nm. Applied to detect trace levels of diuron determined by spectrophotometry, the fluorescent sensor exhibited a good linear response within the range of 0.10-50.0 μg L with a coefficient of determination of 0.9997. The limit of detection was 0.07 μg L. Coupled with a smartphone-assisted fluorescence color analysis platform, the probe produced linearity in the range of 5.0-50.0 μg L, with an R of 0.9986 and a limit of detection of 2.5 μg L. Both developed methods successfully determined diuron in radish, tomato, cucumber and green tea. Recoveries were between 90.5 and 107.3 % with RSDs <8.0 % for the fluorescent sensor coupled with spectrophotometry and 91.5-105.3 % with an RSD of <7 % for the smartphone-assisted method. The reliability and accuracy of both methods were confirmed by comparison with high-performance liquid chromatography. The proposed fluorescent probe coupled with the smartphone-assisted color analysis platform provided high sensitivity and good selectivity while being simple, convenient, rapid and inexpensive. The method has several application possibilities for on-site detection of trace diuron.
使用嵌入分子印迹聚合物（MIP）中的氮掺杂石墨烯量子点（N-GQDs）修饰的多孔氧化锌（ZnO）成功开发了一种荧光探针。通过溶胶-凝胶聚合法合成了ZnO∗N-GQDs∗MIP传感探针，该探针在403nm处表现出强烈的荧光发射。将其应用于分光光度法测定痕量敌草隆，该荧光传感器在0.10-50.0μg/L范围内表现出良好的线性响应，测定系数为0.9997。检测限为0.07μg/L。结合智能手机辅助荧光颜色分析平台，该探针在5.0-50.0μg/L范围内呈现线性，R为0.9986，检测限为2.5μg/L。两种开发的方法均成功测定了萝卜、番茄、黄瓜和绿茶中的敌草隆。对于与分光光度法联用的荧光传感器，回收率在90.5%至107.3%之间，相对标准偏差<8.0%；对于智能手机辅助方法，回收率在91.5%至105.3%之间，相对标准偏差<7%。通过与高效液相色谱法比较，证实了两种方法的可靠性和准确性。所提出的荧光探针与智能手机辅助颜色分析平台相结合，具有高灵敏度和良好的选择性，同时简单、方便、快速且成本低廉。该方法在痕量敌草隆的现场检测中有多种应用可能性。
