Center of Excellence for Innovation in Chemistry, Division of Physical Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.
Division of Physical Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.
Mikrochim Acta. 2023 Mar 11;190(4):129. doi: 10.1007/s00604-023-05704-3.
A nanocomposite fluorescent probe was fabricated for the simultaneous determination of florfenicol and sparfloxacin based on fluorescence quenching. The probe was synthesized by integrating nitrogen-doped graphene quantum dots (N-GQDs), cadmium telluride quantum dots (CdTe QDs) and zinc oxide nanoparticles (ZnO) into a molecularly imprinted polymer (MIP). The determination was based on the quenching of fluorescence emissions from N-GQDs by florfenicol, detected at 410 nm, and the quenching of fluorescence emissions from CdTe QDs by sparfloxacin, detected at 550 nm. The fluorescent probe was highly sensitive and specific with good linear relationships for florfenicol and sparfloxacin in the range 0.10 to 100.0 μg L. The limits of detection for florfenicol and sparfloxacin were 0.06 and 0.10 μg L, respectively. The fluorescent probe was used to determine florfenicol and sparfloxacin in food samples and the results agreed well with the results of chromatographic determination. Recoveries of spiked milk, egg and chicken samples reached 93.3-103.4% with good precision (RSD < 6%). The advantages of the nano-optosensor include high sensitivity and selectivity, simplicity, rapidity, convenience, good accuracy and precision.
一种基于荧光猝灭原理的用于同时测定氟苯尼考和司帕沙星的纳米复合荧光探针被制备。该探针通过将氮掺杂石墨烯量子点（N-GQDs）、碲化镉量子点（CdTe QDs）和氧化锌纳米粒子（ZnO）整合到分子印迹聚合物（MIP）中合成。测定基于氟苯尼考对 N-GQDs 荧光发射的猝灭，在 410nm 处检测，以及司帕沙星对 CdTe QDs 荧光发射的猝灭，在 550nm 处检测。该荧光探针具有高灵敏度和特异性，对氟苯尼考和司帕沙星在 0.10 至 100.0μg L 的范围内具有良好的线性关系。氟苯尼考和司帕沙星的检测限分别为 0.06 和 0.10μg L。该荧光探针用于测定食品样品中的氟苯尼考和司帕沙星，其结果与色谱测定结果吻合良好。牛奶、鸡蛋和鸡肉样品的加标回收率达到 93.3-103.4%，精密度良好（RSD<6%）。该纳米光电传感器的优点包括高灵敏度和选择性、简单、快速、方便、良好的准确性和精密度。
