Marcus L, Margel S, Savin H, Offarim M, Ravid M
Am Heart J. 1985 Jul;110(1 Pt 1):30-9. doi: 10.1016/0002-8703(85)90510-1.
The usefulness of a new biocompatible, specific immunosorbent, Agarose-Polyacrolein Microsphere Beads--Antidigoxin antibodies (APAMB-AD) for hemoperfusive removal of digoxin in digoxin intoxicated dogs is described. The sorbent contains antidigoxin antibodies covalently bound to polyacrolein microspheres, 0.2 micron in diameter. Thousands of microspheres are matrix-encapsulated in cross-linked agarose to form beads 500 to 800 micron in diameter. The sorbent removes digoxin specifically, leaving other components of the blood intact, in contrast to the nonspecific sorbents (charcoal and ion exchange resins) currently in use. Digoxin-intoxicated dogs looked ill, vomited, and their ECGs showed malignant arrhythmias which were reversed during the first hour of hemoperfusion. By 2 hours of hemoperfusion, the ECG tracings returned to the preintoxication state. Up to 27% of the total body digoxin burden was removed. The sorbent is biocompatible. Neither the formed elements nor a battery of the routinely assayed soluble components of the blood or complement (C'4) were altered significantly during the hemoperfusion trials. The dogs tolerated the hemoperfusion well and all survived the intoxication. Nonhemoperfused dogs or dogs whose blood was hemoperfused through beads lacking antidigoxin did not survive the digoxin intoxication.
本文描述了一种新型生物相容性特异性免疫吸附剂——琼脂糖-聚丙烯醛微球珠-抗地高辛抗体（APAMB-AD）用于血液灌流清除地高辛中毒犬体内地高辛的有效性。该吸附剂含有与直径为0.2微米的聚丙烯醛微球共价结合的抗地高辛抗体。数千个微球被基质包封在交联琼脂糖中，形成直径为500至800微米的珠粒。与目前使用的非特异性吸附剂（活性炭和离子交换树脂）不同，该吸附剂能特异性清除地高辛，而使血液中的其他成分保持完整。地高辛中毒的犬表现出病态、呕吐，其心电图显示恶性心律失常，在血液灌流的第一小时内这些症状得到缓解。到血液灌流2小时时，心电图描记恢复到中毒前状态。全身地高辛负荷的27%被清除。该吸附剂具有生物相容性。在血液灌流试验期间，血液中的有形成分、一系列常规检测的可溶性成分或补体（C'4）均未发生明显改变。犬对血液灌流耐受性良好，所有犬均从中毒中存活下来。未进行血液灌流的犬或血液通过缺乏抗地高辛的珠粒进行灌流的犬未能从地高辛中毒中存活。
