Savin H, Michaeli I, Margel S, Ravid M
Department Medicine A, Sapir Medical Center, Sackler School of Medicine, Tel Aviv University, Rehovot, Israel.
Biomater Artif Cells Artif Organs. 1987;15(3):539-47. doi: 10.3109/10731198709117551.
Extracorporeal hemoperfusion through polyacrolein microsphere beads (APAMB) attached to antidigoxin antibodies is an effective treatment of digitalis intoxication. In order to increase the binding capacity of the APAMB columns the active sites of the antidigoxin antibodies were protected by ouabain during the binding to the microbeads. This brought about an 11-18 percent increase in digoxin binding capacity of the columns. It was also found that binding capacity does not increase with the rise of antibody concentration beyond a certain limit. Protection of antibody binding sites and determination of optimal concentration are, therefore necessary steps during preparation of antibody based hemoperfusion columns.
通过附着抗地高辛抗体的聚丙烯醛微球珠（APAMB）进行体外血液灌流是治疗洋地黄中毒的有效方法。为了提高APAMB柱的结合能力，在抗地高辛抗体与微珠结合过程中，用地高辛保护其活性位点。这使得柱的地高辛结合能力提高了11% - 18%。还发现，抗体浓度超过一定限度后，结合能力不会随着抗体浓度的升高而增加。因此，保护抗体结合位点并确定最佳浓度是制备基于抗体的血液灌流柱过程中的必要步骤。
