Li Wenan, Deng Kaishu, Zhang Mengyue, Xu Yan, Zhang Jingxi, Liang Qingsheng, Yang Zhiyou, Jin Leigang, Hu Chuanyin, Zhao Yun-Tao
Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, College of Food Science and Technology, Modern Biochemistry Experimental Center, Zhanjiang Municipal Key Laboratory of Marine Drugs and Nutrition for Brain Health, Guangdong Ocean University, Zhanjiang 524088, China.
State Key Laboratory of Pharmaceutical Biotechnology, Department of Medicine, The University of Hong Kong, Hong Kong SAR, China.
Int J Mol Sci. 2025 Mar 14;26(6):2626. doi: 10.3390/ijms26062626.
Aucubin (AU) is one of the main components of the traditional Chinese medicine Oliv (EU). This study investigated the effects of AU on aging-related skeletal muscle atrophy in vitro and in vivo. The results of network pharmacology revealed the potential therapeutic effects of AU on muscle atrophy. In vitro, AU effectively attenuated D-gal-induced cellular damage, reduced the number of senescence-associated β-galactosidase (SA-β-Gal)-positive cells, down-regulated the expression levels of muscle atrophy-related proteins Atrogin-1 and MuRF1, and improved myotube differentiation, thereby mitigating myotube atrophy. Notably, AU was found to attenuate oxidative stress and apoptosis in skeletal muscle cells by reducing ROS production, regulating Cleaved caspase3 and BAX/Bcl-2 expression in apoptotic pathways, and enhancing Sirt1 and PGC-1α signaling pathways. In vivo studies demonstrated that AU treatment extended the average lifespan of (, increased locomotor activity, improved body wall muscle mitochondrial content, and alleviated oxidative damage in . These findings suggested that AU can ameliorate aging-related muscle atrophy and show significant potential in preventing and treating muscle atrophy.
桃叶珊瑚苷（AU）是传统中药山茱萸（EU）的主要成分之一。本研究调查了AU在体外和体内对衰老相关骨骼肌萎缩的影响。网络药理学结果揭示了AU对肌肉萎缩的潜在治疗作用。在体外，AU有效减轻了D-半乳糖诱导的细胞损伤，减少了衰老相关β-半乳糖苷酶（SA-β-Gal）阳性细胞的数量，下调了肌肉萎缩相关蛋白Atrogin-1和MuRF1的表达水平，并改善了肌管分化，从而减轻了肌管萎缩。值得注意的是，发现AU通过减少活性氧生成、调节凋亡途径中裂解的caspase3和BAX/Bcl-2表达以及增强Sirt1和PGC-1α信号通路来减轻骨骼肌细胞中的氧化应激和凋亡。体内研究表明，AU治疗延长了（此处原文似乎不完整）的平均寿命，增加了运动活性，改善了体壁肌肉线粒体含量，并减轻了（此处原文似乎不完整）中的氧化损伤。这些发现表明，AU可以改善衰老相关的肌肉萎缩，并在预防和治疗肌肉萎缩方面显示出巨大潜力。
