Antonopoulou Georgia, Magkrioti Christiana, Chatzidaki Ismini, Nastos Dimitris, Grammenoudi Sofia, Bozonelos Konstantinos, Aidinis Vassilis
Institute for Fundamental Biomedical Research, Biomedical Sciences Research Center Alexander Fleming, 16672 Athens, Greece.
Int J Mol Sci. 2025 Mar 20;26(6):2811. doi: 10.3390/ijms26062811.
The lysophosphatidic acid receptor 1 (LPAR1) is one of the six cognate G protein-coupled receptors of the bioactive, growth factor-like phospholipid lysophosphatidic acid (LPA). LPAR1 is widely expressed in different cell types and mediates many LPA effects. LPAR1 has been implicated in several chronic inflammatory diseases, and especially pulmonary fibrosis, where it has been established as a promising therapeutic target. Herein, we present the generation of several mouse strains through genetic recombination. These strains include an initial versatile strain (tm1a) from which three other strains derive: an reporter knockout strain (tm1b) where LacZ has replaced exon 3 of ; a "floxed" strain (tm1c), where exon 3 is flanked by two loxP sites allowing conditional, cell-specific inactivation; and a complete KO strain of (tm1d), where exon 3 has been deleted. The generated strains are novel genetic tools, that can have various applications in studying LPA-LPAR1 signaling and its role in normal physiology and disease.
溶血磷脂酸受体1（LPAR1）是生物活性、生长因子样磷脂溶血磷脂酸（LPA）的六种同源G蛋白偶联受体之一。LPAR1在不同细胞类型中广泛表达，并介导许多LPA效应。LPAR1与多种慢性炎症性疾病有关，尤其是肺纤维化，在肺纤维化中它已被确立为一个有前景的治疗靶点。在此，我们通过基因重组展示了几种小鼠品系的产生。这些品系包括一个初始通用品系（tm1a），其他三个品系由此衍生而来：一个报告基因敲除品系（tm1b），其中LacZ取代了[具体基因]的外显子3；一个“floxed”品系（tm1c），其中外显子3两侧有两个loxP位点，允许条件性、细胞特异性失活；以及一个[具体基因]的完全敲除品系（tm1d），其中外显子3已被删除。所产生的品系是新型遗传工具，可在研究LPA-LPAR1信号传导及其在正常生理学和疾病中的作用方面有多种应用。
