• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

镁合金AZ31水热涂层对人骨髓间充质干细胞成骨分化的影响：从基因到蛋白质分析

Effect of Hydrothermal Coatings of Magnesium AZ31 Alloy on Osteogenic Differentiation of hMSCs: From Gene to Protein Analysis.

作者信息

Costa Viviana, Raimondi Lavinia, Scilabra Simone Dario, Pinto Margot Lo, Bellavia Daniele, De Luca Angela, Guglielmi Pasquale, Cusanno Angela, Cattini Luca, Pulsatelli Lia, Pavarini Matteo, Chiesa Roberto, Giavaresi Gianluca

机构信息

CS-Surgical Sciences and Technologies-SS Omics Science Platform for Personalized Orthopedics, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy.

Ri.MED Foundation, IRCCS ISMETT, Via Ernesto Tricomi 5, 90145 Palermo, Italy.

出版信息

Materials (Basel). 2025 Mar 12;18(6):1254. doi: 10.3390/ma18061254.

DOI:10.3390/ma18061254
PMID:40141537
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11944061/
Abstract

An Mg-based alloy device manufactured via a superplastic forming process (Mg-AZ31+SPF) and coated using a hydrothermal method (Mg AZ31+SPF+HT) was investigated as a method to increase mechanical and osteointegration capability. The cell viability and osteointegrative properties of alloy-derived Mg AZ31+SPF and Mg AZ31+SPF+HT extracts were investigated regarding their effect on human mesenchymal stem cells (hMSCs) (maintained in basal (BM) and osteogenic medium (OM)) after 7 and 14 days of treatment. The viability was analyzed through metabolic activity and double-strand DNA quantification, while the osteoinductive effects were evaluated through qRT-PCR, osteoimage, and BioPlex investigations. Finally, a preliminary liquid mass spectrometry analysis was conducted on the secretome of hMSCs. Biocompatibility analysis revealed no toxic effect on cells' viability or proliferation during the experimental period. A modulation effect was observed on the osteoblast pre-commitment genes of hMSCs treated with Mg-AZ31+SPF+HT in OM, which was supported by mineralization nodule analysis. A preliminary mass spectrometry investigation highlighted the modulation of protein clusters involved in extracellular exosomes, Hippo, and the lipid metabolism process. In conclusion, our results revealed that the Mg AZ31+SPF+HT extracts can modulate the canonical and non-canonical osteogenic process in vitro, suggesting their possible application in bone tissue engineering.

摘要

研究了一种通过超塑性成型工艺制造的镁基合金装置（Mg-AZ31+SPF）以及采用水热法涂层后的装置（Mg AZ31+SPF+HT），将其作为提高机械性能和骨整合能力的一种方法。研究了合金衍生的Mg AZ31+SPF和Mg AZ31+SPF+HT提取物对人骨髓间充质干细胞（hMSCs）（在基础培养基（BM）和成骨培养基（OM）中培养）在处理7天和14天后的细胞活力和骨整合特性。通过代谢活性和双链DNA定量分析细胞活力，同时通过qRT-PCR、骨成像和生物芯片研究评估成骨诱导作用。最后，对hMSCs的分泌蛋白组进行了初步的液相质谱分析。生物相容性分析显示，在实验期间对细胞活力或增殖没有毒性作用。在OM中用Mg-AZ31+SPF+HT处理的hMSCs的成骨前体基因上观察到调节作用，矿化结节分析支持了这一点。初步的质谱研究突出了参与细胞外囊泡、Hippo和脂质代谢过程的蛋白质簇的调节作用。总之，我们的结果表明，Mg AZ31+SPF+HT提取物可以在体外调节经典和非经典的成骨过程，表明它们在骨组织工程中可能具有应用价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f16/11944061/22c81e014aaa/materials-18-01254-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f16/11944061/9d470a06fb2a/materials-18-01254-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f16/11944061/7d8ef2bea767/materials-18-01254-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f16/11944061/e764dca9fac1/materials-18-01254-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f16/11944061/e642ed0074ee/materials-18-01254-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f16/11944061/850290631ccd/materials-18-01254-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f16/11944061/e4906aaca5cc/materials-18-01254-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f16/11944061/6fcec45fb380/materials-18-01254-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f16/11944061/d6d8af028ba5/materials-18-01254-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f16/11944061/fab3aac839b3/materials-18-01254-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f16/11944061/22c81e014aaa/materials-18-01254-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f16/11944061/9d470a06fb2a/materials-18-01254-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f16/11944061/7d8ef2bea767/materials-18-01254-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f16/11944061/e764dca9fac1/materials-18-01254-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f16/11944061/e642ed0074ee/materials-18-01254-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f16/11944061/850290631ccd/materials-18-01254-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f16/11944061/e4906aaca5cc/materials-18-01254-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f16/11944061/6fcec45fb380/materials-18-01254-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f16/11944061/d6d8af028ba5/materials-18-01254-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f16/11944061/fab3aac839b3/materials-18-01254-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f16/11944061/22c81e014aaa/materials-18-01254-g010.jpg

相似文献

1
Effect of Hydrothermal Coatings of Magnesium AZ31 Alloy on Osteogenic Differentiation of hMSCs: From Gene to Protein Analysis.镁合金AZ31水热涂层对人骨髓间充质干细胞成骨分化的影响：从基因到蛋白质分析
Materials (Basel). 2025 Mar 12;18(6):1254. doi: 10.3390/ma18061254.
2
Functionalized magnesium alloys obtained by superplastic forming process retain osteoinductive and antibacterial properties: An in-vitro study.通过超塑性成型工艺获得的功能化镁合金保留骨诱导和抗菌性能：一项体外研究。
Dent Mater. 2024 Mar;40(3):557-562. doi: 10.1016/j.dental.2024.01.005. Epub 2024 Feb 7.
3
Corrosion resistance and antibacterial activity of zinc-loaded montmorillonite coatings on biodegradable magnesium alloy AZ31.载锌蒙脱石涂层对可生物降解镁合金 AZ31 的耐腐蚀和抗菌活性。
Acta Biomater. 2019 Oct 15;98:196-214. doi: 10.1016/j.actbio.2019.05.069. Epub 2019 May 31.
4
Effect of High- and Low-Molecular-Weight Hyaluronic-Acid-Functionalized-AZ31 Mg and Ti Alloys on Proliferation and Differentiation of Osteoblast Cells.高分子量和低分子量透明质酸功能化AZ31镁合金及钛合金对成骨细胞增殖和分化的影响
ACS Biomater Sci Eng. 2018 Nov 12;4(11):3874-3884. doi: 10.1021/acsbiomaterials.8b00968. Epub 2018 Oct 1.
5
In vitro and in vivo evaluation of MgF coated AZ31 magnesium alloy porous scaffolds for bone regeneration.用于骨再生的MgF涂层AZ31镁合金多孔支架的体外和体内评价
Colloids Surf B Biointerfaces. 2017 Jan 1;149:330-340. doi: 10.1016/j.colsurfb.2016.10.037. Epub 2016 Oct 22.
6
Enhanced corrosion resistance and cytocompatibility of biomimetic hyaluronic acid functionalised silane coating on AZ31 Mg alloy for orthopaedic applications.用于骨科应用的仿生透明质酸功能化硅烷涂层对 AZ31 Mg 合金增强耐腐蚀性和细胞相容性。
J Mater Sci Mater Med. 2018 Aug 28;29(9):144. doi: 10.1007/s10856-018-6150-5.
7
A cross-linked coating loaded with antimicrobial peptides for corrosion control, early antibacterial, and sequential osteogenic promotion on a magnesium alloy as orthopedic implants.一种负载抗菌肽的交联涂层，用于镁合金骨科植入物的腐蚀控制、早期抗菌和后续成骨促进。
Acta Biomater. 2025 Jan 24;193:604-622. doi: 10.1016/j.actbio.2024.12.046. Epub 2024 Dec 21.
8
Mussel-inspired functionalization of PEO/PCL composite coating on a biodegradable AZ31 magnesium alloy.贻贝启发的可生物降解AZ31镁合金上PEO/PCL复合涂层的功能化处理
Colloids Surf B Biointerfaces. 2016 May 1;141:327-337. doi: 10.1016/j.colsurfb.2016.02.004. Epub 2016 Feb 4.
9
Rapid coating of AZ31 magnesium alloy with calcium deficient hydroxyapatite using microwave energy.利用微波能量快速涂覆 AZ31 镁合金的欠钙羟基磷灰石。
Mater Sci Eng C Mater Biol Appl. 2015 Apr;49:364-372. doi: 10.1016/j.msec.2015.01.046. Epub 2015 Jan 10.
10
Micromorphological effect of calcium phosphate coating on compatibility of magnesium alloy with osteoblast.磷酸钙涂层对镁合金与成骨细胞相容性的微观形态学影响
Sci Technol Adv Mater. 2017 Jan 23;18(1):96-109. doi: 10.1080/14686996.2016.1266238. eCollection 2017.

引用本文的文献

1
Hydrothermal Magnesium Alloy Extracts Modulate MicroRNA Expression in RAW264.7 Cells: Implications for Bone Remodeling.水热镁合金提取物调节RAW264.7细胞中的微小RNA表达：对骨重塑的影响
J Funct Biomater. 2025 Aug 21;16(8):303. doi: 10.3390/jfb16080303.

本文引用的文献

1
Hyperglycemia exerts disruptive effects on the secretion of TGF-β and its matrix ligands, decorin and biglycan, by mesenchymal sub-populations and macrophages during bone repair.高血糖对骨修复过程中间充质亚群和巨噬细胞分泌转化生长因子-β（TGF-β）及其基质配体核心蛋白聚糖和双糖链蛋白聚糖具有破坏作用。
Front Dent Med. 2023 Jun 26;4:1200122. doi: 10.3389/fdmed.2023.1200122. eCollection 2023.
2
Towards Accurate Biocompatibility: Rethinking Cytotoxicity Evaluation for Biodegradable Magnesium Alloys in Biomedical Applications.迈向精确的生物相容性：重新思考生物医学应用中可降解镁合金的细胞毒性评估
J Funct Biomater. 2024 Dec 18;15(12):382. doi: 10.3390/jfb15120382.
3
Induced pluripotent stem cell-derived mesenchymal stem cells reverse bleomycin-induced pulmonary fibrosis and related lung stiffness.
诱导多能干细胞衍生的间充质干细胞逆转博来霉素诱导的肺纤维化及相关肺硬度。
Biomed Pharmacother. 2024 Sep;178:117259. doi: 10.1016/j.biopha.2024.117259. Epub 2024 Aug 7.
4
Exosomes: A New Hope for Angiogenesis-Mediated Bone Regeneration.外泌体：血管生成介导骨再生的新希望。
Int J Mol Sci. 2024 May 10;25(10):5204. doi: 10.3390/ijms25105204.
5
Exploring the Implications of Golgi Apparatus Dysfunction in Bone Diseases.探索高尔基体功能障碍在骨疾病中的影响。
Cureus. 2024 Mar 26;16(3):e56982. doi: 10.7759/cureus.56982. eCollection 2024 Mar.
6
Functionalized magnesium alloys obtained by superplastic forming process retain osteoinductive and antibacterial properties: An in-vitro study.通过超塑性成型工艺获得的功能化镁合金保留骨诱导和抗菌性能：一项体外研究。
Dent Mater. 2024 Mar;40(3):557-562. doi: 10.1016/j.dental.2024.01.005. Epub 2024 Feb 7.
7
Magnesium Alloys in Orthopedics: A Systematic Review on Approaches, Coatings and Strategies to Improve Biocompatibility, Osteogenic Properties and Osteointegration Capabilities.骨科用镁合金：改善生物相容性、成骨特性和骨整合能力的方法、涂层和策略的系统评价。
Int J Mol Sci. 2023 Dec 24;25(1):282. doi: 10.3390/ijms25010282.
8
Lipolysis supports bone formation by providing osteoblasts with endogenous fatty acid substrates to maintain bioenergetic status.脂肪分解通过为成骨细胞提供内源性脂肪酸底物来维持生物能量状态，从而支持骨形成。
Bone Res. 2023 Nov 24;11(1):62. doi: 10.1038/s41413-023-00297-2.
9
Fucoidan/collagen composite coating on magnesium alloy for better corrosion resistance and pro-endothelialization potential.藻酸盐/胶原蛋白复合涂层在镁合金上，以提高耐腐蚀性和促进内皮化潜力。
Int J Biol Macromol. 2024 Jan;255:128044. doi: 10.1016/j.ijbiomac.2023.128044. Epub 2023 Nov 20.
10
Long-Term Temporospatial Complementary Relationship between Degradation and Bone Regeneration of Mg-Al Alloy.镁铝合金降解与骨再生的长期时空间互补关系。
ACS Appl Bio Mater. 2023 Nov 20;6(11):4703-4713. doi: 10.1021/acsabm.3c00488. Epub 2023 Oct 22.