Technical and Performance Characteristics Between Different Anti-Mullerian Hormone (AMH) Assay Methods and Antral Follicle Count (AFC) in Malaysian Women with Infertility: A University-Based Centre Cohort.

BACKGROUNDS

Evaluating the AMH level and AFC are crucial in infertility practice. Thus, accurate measurement is paramount. Various methods are available and selecting the right process is essential to ensure good reproducibility and precise results. Thus, our study aims to determine the analytical performance of AMH Plus and AFIAS-AMH and the correlation between the two AMH assays and AFC values.

METHODS

A prospective study was conducted at the Advanced Reproductive Center, Hospital Canselor Tuanku Mukhriz (HCTM), Faculty of Medicine, National University of Malaysia, to compare the correlation between the AMH assay methods and AFCs. It included 100 patients from Feb 2024 until June 2024.

RESULTS

A total of 100 women with infertility, with a mean age of 35.87 ± 3.92, were included in our study. Our results revealed a strong positive correlation between the two AMH assays, with the comparable performance of AMH Plus and AFIAS-AMH. We also found that the serum AMH evaluation was comparable with the routine AFC assessment.

CONCLUSIONS

Our findings indicated that serum AMH levels determine the association between AMH levels and follicle counts and the correlation between AMH concentrations and assays. Our study demonstrated the competent repeatability, acceptable linearity, and laboratory precision of the AFIAS-AMH test and comparative assessment of the AFC and serum AMH evaluation.