Lakmuang Chayaporn, Ibrahim Syahriar Nur Maulana Malik, Kaewjon Teeratat, Kraisitudomsook Nattapol, Somboonna Naraporn, Chanabun Ratmanee, Chindamporn Ariya, Pombubpa Nuttapon
Bioinformatics and Computational Biology Program, Graduate School, Chulalongkorn University, Bangkok 10330, Thailand.
Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Life (Basel). 2025 Mar 18;15(3):488. doi: 10.3390/life15030488.
Fungal pathogens prevalent in agricultural areas pose a significant risk to human health, with some exhibiting high fatality rates, as reported by the WHO Fungal Pathogen Priority List (WHO FPPL). This study investigates fungal communities in northeast Thailand's agricultural areas, focusing on potential reservoirs of the WHO FPPL. Samples were collected from rice, cassava, rubber trees, and sugarcane fields across 18 provinces with distinct geological features. Metabarcoding of the ITS1 region and taxonomic analysis were conducted, and potential pathogens were selected according to WHO FPPL criteria. The results showed that overall fungal community richness and diversity were influenced by plant fields but not significantly different by geological features. Soil organic matter and water content affected fungal dynamics only in rubber tree fields. Fungal pathogens from the WHO FPPL were found in all four plant fields, with higher abundance in Chaiyaphum province, especially in sugarcane fields, and the lowest in Nong Bua Lam Phu. , a high-priority pathogen, was predominantly associated with rock salt features. This study underscores the need for vigilance among farmers and emphasizes the importance of confirming fungal pathogenicity.
世界卫生组织真菌病原体优先清单（WHO FPPL）报告称，农业地区普遍存在的真菌病原体对人类健康构成重大风险，其中一些病原体的致死率很高。本研究调查了泰国东北部农业地区的真菌群落，重点关注WHO FPPL中的潜在病原体宿主。从18个具有不同地质特征的省份的稻田、木薯田、橡胶园和甘蔗田采集样本。对ITS1区域进行了代谢条形码分析和分类学分析，并根据WHO FPPL标准选择了潜在病原体。结果表明，真菌群落的总体丰富度和多样性受农田影响，但不受地质特征的显著影响。土壤有机质和含水量仅在橡胶园影响真菌动态。在所有四个农田中都发现了来自WHO FPPL的真菌病原体，猜也蓬府的丰度更高，尤其是在甘蔗田中，农布拉姆普府的丰度最低。高优先级病原体 主要与岩盐特征有关。本研究强调了农民保持警惕的必要性，并强调了确认真菌致病性的重要性。
