Rapid Detection of Typhimurium During Cell Attachment on Three Food-Contact Surfaces Using Long-Read Sequencing.

spp. are pathogenic microorganisms linked to foodborne outbreaks associated with eggs and egg products. can resist sanitation of egg processing equipment and form biofilms on food-contact surfaces. A major challenge for controlling is the ability to detect the cells during the early stages of attachment to indicate that interventions are needed to sanitize the surface. This research investigated the use of long-read sequencing to identify during the early stages (0-5 h) of cell attachment to three common food-contact surfaces-stainless steel, silicone, and nylon-and compared it with traditional microbiological methods. Results of the conventional plate counts showed that the detection of began after three hours of incubation, with less than 1 log CFU/cm of growth. Silicone had the highest number of attached (0.87 log CFU/cm), followed by stainless steel (0.70 log CFU/cm). Long-read whole genome sequencing identified attached on stainless steel, silicone, and nylon after only one hour of incubation. The results of this study suggest that long-read sequencing could be a very useful method for detecting at low concentrations in the processing environment during the early stages of cell attachment to food-contact surfaces, allowing for correct sanitation intervention.