Bermudez-Aguirre Daniela, Tilman Shannon, Uknalis Joseph, Niemira Brendan A, Counihan Katrina L
Characterization and Interventions for Foodborne Pathogens Research Unit, Eastern Regional Research Center, United States Department of Agriculture, Agricultural Research Service, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA.
Microbial and Chemical Food Safety Research Unit, Eastern Regional Research Center, United States Department of Agriculture, Agricultural Research Service, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA.
Microorganisms. 2025 Feb 28;13(3):548. doi: 10.3390/microorganisms13030548.
spp. are pathogenic microorganisms linked to foodborne outbreaks associated with eggs and egg products. can resist sanitation of egg processing equipment and form biofilms on food-contact surfaces. A major challenge for controlling is the ability to detect the cells during the early stages of attachment to indicate that interventions are needed to sanitize the surface. This research investigated the use of long-read sequencing to identify during the early stages (0-5 h) of cell attachment to three common food-contact surfaces-stainless steel, silicone, and nylon-and compared it with traditional microbiological methods. Results of the conventional plate counts showed that the detection of began after three hours of incubation, with less than 1 log CFU/cm of growth. Silicone had the highest number of attached (0.87 log CFU/cm), followed by stainless steel (0.70 log CFU/cm). Long-read whole genome sequencing identified attached on stainless steel, silicone, and nylon after only one hour of incubation. The results of this study suggest that long-read sequencing could be a very useful method for detecting at low concentrations in the processing environment during the early stages of cell attachment to food-contact surfaces, allowing for correct sanitation intervention.
某些特定物种是与鸡蛋及蛋制品相关的食源性疾病暴发的致病微生物。它们能够抵抗蛋加工设备的卫生处理，并在食品接触表面形成生物膜。控制这些微生物的一个主要挑战在于，在其附着的早期阶段检测细胞的能力，以表明需要采取干预措施对表面进行卫生处理。本研究调查了使用长读长测序技术在细胞附着到三种常见食品接触表面（不锈钢、硅胶和尼龙）的早期阶段（0 - 5小时）识别这些微生物的情况，并将其与传统微生物学方法进行比较。传统平板计数的结果表明，孵育三小时后才开始检测到这些微生物，生长量小于1 log CFU/cm²。硅胶表面附着的这些微生物数量最多（0.87 log CFU/cm²），其次是不锈钢（0.70 log CFU/cm²）。长读长全基因组测序在孵育仅一小时后就识别出了不锈钢、硅胶和尼龙表面附着的这些微生物。本研究结果表明，长读长测序可能是一种非常有用的方法，用于在细胞附着到食品接触表面的早期阶段检测加工环境中低浓度的这些微生物，从而实现正确的卫生干预。
